PREPARATION OF SUSPENSIONS OF HUMAN CERVICAL EPITHELIAL-CELLS SUITABLE FOR BIOCHEMICAL, CYTOCHEMICAL AND IMMUNOCYTOCHEMICAL INVESTIGATIONS
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- Published online on: February 1, 1993 https://doi.org/10.3892/ijo.2.2.233
- Pages: 233-236
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Abstract
A simple gradient centrifuging method that separates epithelial cells from suspensions of material obtained by conventional cervical sampling procedures, is described. Heterogeneous cell suspensions were disaggregated by syringing and placed on a discontinuous density gradient. Following centrifugation up to 5 bands of, cells could be collected. The three central bands of epithelial cells corresponded to a density range between 1.035g/ml and 1.055g/ml. The cell samples so obtained are suitable for biochemical, cytochemical and immunocytochemical investigations.