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SECRETION AND BINDING OF INSULIN-LIKE GROWTH FACTOR-I BY A HUMAN MEDULLARY-THYROID CARCINOMA CELL-LINE

  • Authors:
    • CR BETHEL
    • HF SAADI
    • WM HOUT
    • JC VITULLO
    • DC ARON

  • View Affiliations

  • Published online on: June 1, 1993     https://doi.org/10.3892/ijo.2.6.961
  • Pages: 961-967
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Abstract

The TT human medullary thyroid carcinoma cell line secretes a 7.5 kD species with insulin-like growth factor (IGF-I) immunoreactivity and HPLC mobility and expresses IGF-I mRNA. Conditioned medium also contained IGF-binding activity with a pattern of displacement of [I-125]-IGF-I characteristic of IGF binding proteins. Western ligand analysis and immunobloting with anti-IGFBP4 antiserum identified a 34 kD IGF-I binding species. Northern analysis identified a 2.1 kb IGFBP4 mRNA species. Cell surface binding of [I-125]-IGF-I identified Type I IGF receptors. TT cells constitute a useful model to study the IGF-I autocrine system in the transformed state.

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June 1993
Volume 2 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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  • Impact Factor: 4.5
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  • CiteScore: 9.6
  • CiteScore Rank: Q1: #66/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.88
  • SCImago Journal Rank (SJR): 1.099
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Spandidos Publications style
BETHEL C, SAADI H, HOUT W, VITULLO J and ARON D: SECRETION AND BINDING OF INSULIN-LIKE GROWTH FACTOR-I BY A HUMAN MEDULLARY-THYROID CARCINOMA CELL-LINE. Int J Oncol 2: 961-967, 1993.
APA
BETHEL, C., SAADI, H., HOUT, W., VITULLO, J., & ARON, D. (1993). SECRETION AND BINDING OF INSULIN-LIKE GROWTH FACTOR-I BY A HUMAN MEDULLARY-THYROID CARCINOMA CELL-LINE. International Journal of Oncology, 2, 961-967. https://doi.org/10.3892/ijo.2.6.961
MLA
BETHEL, C., SAADI, H., HOUT, W., VITULLO, J., ARON, D."SECRETION AND BINDING OF INSULIN-LIKE GROWTH FACTOR-I BY A HUMAN MEDULLARY-THYROID CARCINOMA CELL-LINE". International Journal of Oncology 2.6 (1993): 961-967.
Chicago
BETHEL, C., SAADI, H., HOUT, W., VITULLO, J., ARON, D."SECRETION AND BINDING OF INSULIN-LIKE GROWTH FACTOR-I BY A HUMAN MEDULLARY-THYROID CARCINOMA CELL-LINE". International Journal of Oncology 2, no. 6 (1993): 961-967. https://doi.org/10.3892/ijo.2.6.961