Characterization of a novel mouse monoclonal antibody, clone 1E8.33, highly specific for human procollagen 11A1, a tumor-associated stromal component

García-Ocaña,Marcos; Vázquez,Fernando; García-Pravia,Carmen; Fuentes-Martínez,Nelson; Menéndez-Rodríguez,Primitiva; Fresno-Forcelledo,Florentino; Barneo-Serra,Luis; Del Amo-Iribarren,Jokin; Simón-Buela,Laureano; De Los Toyos,Juan  R.

doi:10.3892/ijo.2012.1360

Characterization of a novel mouse monoclonal antibody, clone 1E8.33, highly specific for human procollagen 11A1, a tumor-associated stromal component

Authors:
- Marcos García-Ocaña
- Fernando Vázquez
- Carmen García-Pravia
- Nelson Fuentes-Martínez
- Primitiva Menéndez-Rodríguez
- Florentino Fresno-Forcelledo
- Luis Barneo-Serra
- Jokin Del Amo-Iribarren
- Laureano Simón-Buela
- Juan R. De Los Toyos
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Affiliations: Biotechnological and Biomedical Assays Unit, Technical-Scientific Services, Oviedo University, 33006 Oviedo, Spain, Immunology Area, School of Medicine and Health Sciences, Oviedo University, 33006 Oviedo, Spain, Oncology University Institute of Principado de Asturias (IUOPA), Oviedo University, 33006 Oviedo, Spain, Pathological Anatomy Service, Central University Hospital of Asturias (HUCA), 33006 Oviedo, Spain, Progenika Biopharma, S.A.,48160 Vizcaya, Spain, Oncomatrix S.L., Science and Technology Park of Zamudio, Derio, 48160 Vizcaya, Spain
Published online on: February 7, 2012 https://doi.org/10.3892/ijo.2012.1360
Pages: 1447-1454

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Abstract

A novel IgG1, κ mouse monoclonal antibody (clone 1E8.33) to human procollagen 11A1 has been generated. This antibody is poorly mutated, essentially in germ line configuration; its complementarity determining regions (CDRs) are especially rich in tyrosine and serine residues. The epitope recognized is encompassed in the YNYGTMESYQTEAPR amino acid stretch within the variable region of human procollagen 11A1. Human procollagens 5A1 and 11A1 are very similar. However, this antibody does not cross-react with human procollagen 5A1. In human breast tumors, only the activated peritumoral myofibroblasts show a strong intracytoplasmic staining with this antibody. As procollagen 11A1 is overexpressed in the stroma of human tumors with desmoplastic reaction, this antibody represents a valuable tool for diagnostic purposes.