Cytokine-like 1 is involved in the growth and metastasis of neuroblastoma cells

Wen,Mingjie; Wang,Huanmin; Zhang,Xulong; Long,Jun; Lv,Zhe; Kong,Qingli; An,Yunqing

doi:10.3892/ijo.2012.1552

Cytokine-like 1 is involved in the growth and metastasis of neuroblastoma cells

Authors:
- Mingjie Wen
- Huanmin Wang
- Xulong Zhang
- Jun Long
- Zhe Lv
- Qingli Kong
- Yunqing An
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Affiliations: Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, P.R. China, Department of Surgery, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China
Published online on: July 13, 2012 https://doi.org/10.3892/ijo.2012.1552
Pages: 1419-1424

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Abstract

Cytokine-like 1 (CYTL1) was first identified in CD34+ cells derived from bone marrow and cord blood. The biological functions of CYTL1 remain largely unknown. Here, we reveal a relationship between CYTL1 expression and the biological characteristics of neuroblastoma (NB). The expression of CYTL1 was detected in 10 human tumor cell lines and human NB tissues by RT-PCR and real-time PCR. The inhibitory effect of CYTL1 knockdown on the proliferation, migration and invasion of SH-SY5Y human neuroblastoma cells was studied using the CCK-8 assay and Transwell chamber assays. Among the 10 human tumor cell lines that we examined, CYTL1 was expressed only in SH-SY5Y human neuroblastoma cells. Furthermore, we also observed high levels of CYTL1 expression in human NB tissues. When CYTL1 expression was blocked by siRNA, SH-SY5Y cells showed decreased proliferation, migration and invasion activities. Taken together, our results showed the first evidence of CYTL1 expression in SH-SY5Y neuroblastoma cells and human NB tissues, revealed a possible link between CYTL1 and NB development, and suggested CYTL1 as a potential therapeutic target and diagnosis biomarker for NB.

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