Highly lymphatic metastatic pancreatic cancer cells possess stem cell-like properties

Luo,Guopei; Long,Jiang; Cui,Xiaobo; Xiao,Zhiwen; Liu,Zuqiang; Shi,Si; Liu,Liang; Liu,Chen; Xu,Jin; Li,Min; Yu,Xianjun

doi:10.3892/ijo.2013.1780

Highly lymphatic metastatic pancreatic cancer cells possess stem cell-like properties

Authors:
- Guopei Luo
- Jiang Long
- Xiaobo Cui
- Zhiwen Xiao
- Zuqiang Liu
- Si Shi
- Liang Liu
- Chen Liu
- Jin Xu
- Min Li
- Xianjun Yu
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Affiliations: Department of Pancreatic and Hepatobiliary Surgery, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University; Pancreatic Cancer Institute, Fudan University, Shanghai 200032, P.R. China, The Vivian L. Smith Department of Neurosurgery, The University of Texas Medical School at Houston, Houston, TX 77030, USA
Published online on: January 18, 2013 https://doi.org/10.3892/ijo.2013.1780
Pages: 979-984

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Abstract

Cancer stem cells are thought to be the origin of tumor metastasis. However, evidence of cancer stem cells as the source of lymphatic metastasis in pancreatic cancer is not clear. In this study, we examined the stem cell-like properties of the highly lymphatic metastatic pancreatic cancer cells BxPC-3-LN. Compared with the parental BxPC-3 cells, the BxPC-3-LN cells showed stem cell-like properties, including high lymphatic metastasis potential, self-renewal ability and chemoresistance. In addition, the BxPC-3-LN cells also expressed higher levels of sonic hedgehog and migrating cancer stem cell surface markers (CD133 and CXCR4) compared to the parental BxPC-3 cells. The growth of BxPC-3-LN cells was significantly inhibited by gemcitabine combined with the sonic hedgehog inhibitor cyclopamine. The BxPC-3-LN cells expressed lower levels of let-7, miR-34, miR-107, miR-125, miR-128, miR-130, miR-132 and miR-141 than the parental BxPC-3 cells detected by microRNA PCR array, which were reported to have close relation to stem cell factors. This study provides evidence that cancer stem cells are the major sources of pancreatic cancer lymphatic metastasis, and microRNAs may regulate lymphatic metastasis in pancreatic cancer through modulating cancer stem cells.

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