Selenium improves stem cell potency by stimulating the proliferation and active migration of 3T3-L1 preadipocytes

Park,Shin-Hyung; Kim,Jeong-Hwan; Nam,Soo-Wan; Kim,Byung-Woo; Kim,Gi-Young; Kim,Wun-Jae; Choi,Yung Hyun

doi:10.3892/ijo.2013.2182

Selenium improves stem cell potency by stimulating the proliferation and active migration of 3T3-L1 preadipocytes

Authors:
- Shin-Hyung Park
- Jeong-Hwan Kim
- Soo-Wan Nam
- Byung-Woo Kim
- Gi-Young Kim
- Wun-Jae Kim
- Yung Hyun Choi
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Affiliations: Department of Pathology, Dongeui University College of Oriental Medicine, Busan 614-052, Republic of Korea, Blue-Bio Industry RIC, Dongeui University, Busan 614-714, Republic of Korea, Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea, Department of Urology and Personalized Tumor Engineering Research Center, College of Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea, Department of Biochemistry, Dongeui University College of Oriental Medicine, Busan 614-052, Republic of Korea
Published online on: November 15, 2013 https://doi.org/10.3892/ijo.2013.2182
Pages: 336-342

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Abstract

Selenium is a trace nutrient element that protects cells against oxidative damage. In this study, the potential of selenium to improve stem cell potency through active proliferation and migration of 3T3-L1 preadipocytes was investigated, together with the underlying molecular mechanisms. The results indicated that selenium applied for 24 h stimulated cell proliferation up to 20% compared to untreated control cells. Selenium induced the expression of cyclin-dependent kinase (CDK) 1 and CDK2, which are known to regulate G2/M progression, and significantly downregulated the CDK inhibitors p21 and p27. Selenium also activated the expression of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway, as well as extracellular signal-regulated kinase (ERK). Although LY294002, an inhibitor of PI3K, significantly inhibited the selenium-induced cell proliferation of the 3T3-L1 preadipocytes, PD98059, an inhibitor of ERK, did not affect selenium-induced active proliferation. These results clearly indicate that selenium stimulated cell proliferation through cell cycle progression and PI3K/Akt activation, but not through ERK activation. Furthermore, selenium increased 3T3-L1 cell migration, which was associated with the induction of matrix metalloproteinase (MMP)-2 and MMP-9. Taken together, the current findings suggest that selenium can stimulate stem cell potency by increasing the proliferation and active migration of 3T3-L1 cells.

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