Downregulation of VEGF expression attenuates malignant biological behavior of C6 glioma stem cells

Li,Qingquan; Qiao,Guanqun; Ma,Jun; Li,Yingbin

doi:10.3892/ijo.2014.2331

Downregulation of VEGF expression attenuates malignant biological behavior of C6 glioma stem cells

Authors:
- Qingquan Li
- Guanqun Qiao
- Jun Ma
- Yingbin Li
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Affiliations: Department of Neurosurgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210000, Jiangsu, P.R. China
Published online on: March 10, 2014 https://doi.org/10.3892/ijo.2014.2331
Pages: 1581-1588

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Abstract

Several lines of direct evidence show that gliomas express high levels of vascular endothelial growth factor (VEGF). VEGF can promote the growth of gliomas through angiogenesis. It is believed that gliomas originate in the brain tumor stem cells (BTSCs). However, the direct effect of VEGF on the biological behavior of BTSCs has not been completely elucidated. In this study, we established C6 glioma stem cells (C6GSCs) from the C6 glioma cells. Furthermore, we suppressed the VEGF expression of C6GSCs using lentiviral vector-VEGF shRNA. After transfection, the VEGF expression of C6GSCs was downregulated significantly. The proliferation and invasion capacity of transfected C6GSCs was impaired and the ability of differentiation was enhanced. The data demonstrate that downregulation of VEGF expression attenuates malignant biological behavior of C6GSCs. RNA interference of VEGF expression implies an effective anti-gliomas strategy.

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