Inonotus obliquus-derived polysaccharide inhibits the migration and invasion of human non-small cell lung carcinoma cells via suppression of MMP-2 and MMP-9

Lee,Ki Rim; Lee,Jong Seok; Song,Jeong Eun; Ha,Suk Jin; Hong,Eock Kee

doi:10.3892/ijo.2014.2685

Inonotus obliquus-derived polysaccharide inhibits the migration and invasion of human non-small cell lung carcinoma cells via suppression of MMP-2 and MMP-9

Authors:
- Ki Rim Lee
- Jong Seok Lee
- Jeong Eun Song
- Suk Jin Ha
- Eock Kee Hong
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Affiliations: Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Gangwon-do 200-701, Republic of Korea
Published online on: September 30, 2014 https://doi.org/10.3892/ijo.2014.2685
Pages: 2533-2540

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Abstract

Polysaccharides isolated from the fruiting body of Inonotus obliquus (PFIO) are known to possess various pharmacological properties including antitumor activity. However, the anti-metastatic effect and its underlying mechanistic signaling pathway involved these polysaccharides in human non-small cell lung carcinoma remain unknown. The present study therefore aimed to determine the anti-metastatic potential and signaling pathways of PFIO in the highly metastatic A549 cells. We found that PFIO suppressed the migration and invasive ability of A549 cells while decreasing the expression levels and activity of matrix metalloproteinase (MMP)-2 and MMP-9. Furthermore, PFIO decreased the phosphorylation levels of mitogen-activated protein kinases (MAPKs) and phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) as well as the expression level of COX-2, and inhibited the nuclear translocation of nuclear factor κB (NF-κB) in A549 cells. These results suggested that PFIO could suppress the invasion and migration of human lung carcinoma by reducing the expression levels and activity of MMP-2 and MMP-9 via suppression of MAPKs, PI3K/AKT, and NF-κB signaling pathways.

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