Overexpression of miR‑145 in U87 cells reduces glioma cell malignant phenotype and promotes survival after in vivo implantation

  • Authors:
    • Yong Lu
    • Michael Chopp
    • Xuguang Zheng
    • Mark Katakowski
    • Ding Wang
    • Elise Fraser
    • Monique Nguyen
    • Feng Jiang
  • View Affiliations

  • Published online on: December 23, 2014     https://doi.org/10.3892/ijo.2014.2807
  • Pages: 1031-1038
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Abstract

In the present study, we sought to elucidate the effect of miR‑145 on glioma cell progression and its mechanisms of action. We examined the effects of miR‑145 on proliferation and invasion of U87 glioma cells and on capillary tube formation. Our data show that restoration of miR‑145 in U87 glioma cells significantly reduced their in vitro proliferation, invasion and angiogenesis. However, decreased miR‑145 expression promoted U87 glioma cell proliferation, invasion and angiogenesis, and reduced-expression of miR‑145 increased ADAM17 and EGFR expression in U87 cells. Overexpression of miR‑145 reduced ADAM17 and EGFR expression. VEGF secretion and VEGF expression were decreased by increased miR‑145 expression in U87 cells and were reversed by miR‑145 downregulation in vitro. Nude mice with intracerebral implantation of U87 overexpressing miR‑145 cells exhibited significantly reduced tumor growth and promoted survival compared with control groups. Taken together, these results suggest a role for miR‑145 as a tumor suppressor which inhibits glioma cell proliferation, invasion and angiogenesis in vitro and reduces glioma growth in vivo.
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March-2015
Volume 46 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lu Y, Chopp M, Zheng X, Katakowski M, Wang D, Fraser E, Nguyen M and Jiang F: Overexpression of miR‑145 in U87 cells reduces glioma cell malignant phenotype and promotes survival after in vivo implantation. Int J Oncol 46: 1031-1038, 2015.
APA
Lu, Y., Chopp, M., Zheng, X., Katakowski, M., Wang, D., Fraser, E. ... Jiang, F. (2015). Overexpression of miR‑145 in U87 cells reduces glioma cell malignant phenotype and promotes survival after in vivo implantation. International Journal of Oncology, 46, 1031-1038. https://doi.org/10.3892/ijo.2014.2807
MLA
Lu, Y., Chopp, M., Zheng, X., Katakowski, M., Wang, D., Fraser, E., Nguyen, M., Jiang, F."Overexpression of miR‑145 in U87 cells reduces glioma cell malignant phenotype and promotes survival after in vivo implantation". International Journal of Oncology 46.3 (2015): 1031-1038.
Chicago
Lu, Y., Chopp, M., Zheng, X., Katakowski, M., Wang, D., Fraser, E., Nguyen, M., Jiang, F."Overexpression of miR‑145 in U87 cells reduces glioma cell malignant phenotype and promotes survival after in vivo implantation". International Journal of Oncology 46, no. 3 (2015): 1031-1038. https://doi.org/10.3892/ijo.2014.2807