Effect of HIF-1α on biological activation of human tongue squamous cell carcinoma SCC-15 cells in vitro

Zhou,Xiaokang; Huang,Danqing; Xue,Zhongxiu; Xu,Xiuhui; Wang,Kai; Sun,Yao; Kang,Feiwu

doi:10.3892/ijo.2015.2934

Effect of HIF-1α on biological activation of human tongue squamous cell carcinoma SCC-15 cells in vitro

Authors:
- Xiaokang Zhou
- Danqing Huang
- Zhongxiu Xue
- Xiuhui Xu
- Kai Wang
- Yao Sun
- Feiwu Kang
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Affiliations: Laboratory of Oral Biomedical Science and Translational Medicine, School of Stomatology, Tongji University, Middle Yanchang Road 399, Shanghai 200072, P.R. China
Published online on: March 26, 2015 https://doi.org/10.3892/ijo.2015.2934
Pages: 2346-2354
Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Hypoxia-inducible factor-1α (HIF-1α) is a key regulator for tumor cells and tissues to adapt to hypoxic condition. Suppressing the expression of HIF-1α is important to evaluate its effect on cancer cells. This study was carried out to analyze the effect of HIF-1α on the biological activation of human tongue squamous cell carcinoma (TSCC) SCC-15 cells. In this experiment, deferoxamine mesylate (DFO) was used to induce hypoxic condition. HIF-1α gene was suppressed by lentiviral vector. The effect of the level of HIF-1α expression was tested on the proliferation, cell cycle, cell apoptosis and cell invasion of SCC-15 cells. We demonstrated that SCC-15 cells showed a more aggressive phenotype after treated with DFO. Additionally, DFO was able to induce the expression of HIF-1α protein. Lentiviral vector can effectively inhibit HIF-1α expression on mRNA and protein level. Under normoxic or hypoxic conditions, downregulation of HIF-1α for SCC-15 cells induced cell apoptosis and inhibited growth and invasion. These results showed that suppressing the expression of HIF-1α inhibited the aggressive potential of SCC-15 cells under normoxic and hypoxic condition. Thus, finding an effective and safe pathway to inhibit the expression of HIF-1α can help us to improve the survival rate of human TSCC patients.

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