The anticancer mechanism of 2'-hydroxycinnamaldehyde in human head and neck cancer cells

  • Authors:
    • Sang-Gun Ahn
    • Young-Hee Jin
    • Jung-Hoon Yoon
    • Soo-A Kim
  • View Affiliations

  • Published online on: September 8, 2015     https://doi.org/10.3892/ijo.2015.3152
  • Pages: 1793-1800
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Abstract

Cinnamaldehyde has been shown to effectively induce apoptosis in a number of human cancer cells. In the present study, cinnamaldehyde derivative-induced apoptosis and its signaling pathways were assessed in p53-wild (SGT) and p53-mutant (YD-10B) human head and neck cancer cells. The cinnamaldehyde derivatives, 2'-hydroxycinnamaldehyde (HCA) and 2'-benzoyloxycinnamaldehyde (BCA), exhibited powerful anti-proliferative effects on SGT and YD-10B cells. The apoptotic effect induced by HCA or BCA was supported by caspase-3, -7, -9 and PARP activation, and confirmed by Annexin V-FITC/PI double staining. HCA induced the expression of p21 in both SGT and YD-10B cells. Furthermore, HCA induced the level of pro-apoptotic Bak1 expression while decreasing the level of anti-apoptotic Bcl-2 in both cell lines, suggesting that HCA induced the cell death pathway in a p53-independent manner. HCA also induced the expression of LC3B in SGT and YD-10B cells. Following pre-incubation with the autophagy inhibitor 3-MA, HCA-induced apoptosis was largely increased in SGT cells, while inhibited in YD-10B cells, suggesting that autophagy may actively contribute to HCA-induced apoptosis. Taken together, these observations suggest that HCA may be an effective therapeutic agent in the treatment of head and neck cancer regardless of p53 status.
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November-2015
Volume 47 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ahn S, Jin Y, Yoon J and Kim S: The anticancer mechanism of 2'-hydroxycinnamaldehyde in human head and neck cancer cells. Int J Oncol 47: 1793-1800, 2015.
APA
Ahn, S., Jin, Y., Yoon, J., & Kim, S. (2015). The anticancer mechanism of 2'-hydroxycinnamaldehyde in human head and neck cancer cells. International Journal of Oncology, 47, 1793-1800. https://doi.org/10.3892/ijo.2015.3152
MLA
Ahn, S., Jin, Y., Yoon, J., Kim, S."The anticancer mechanism of 2'-hydroxycinnamaldehyde in human head and neck cancer cells". International Journal of Oncology 47.5 (2015): 1793-1800.
Chicago
Ahn, S., Jin, Y., Yoon, J., Kim, S."The anticancer mechanism of 2'-hydroxycinnamaldehyde in human head and neck cancer cells". International Journal of Oncology 47, no. 5 (2015): 1793-1800. https://doi.org/10.3892/ijo.2015.3152