SCTR regulates cell cycle-related genes toward anti-proliferation in normal breast cells while having pro-proliferation activity in breast cancer cells

  • Authors:
    • Seongeun Kang
    • Byungtak Kim
    • Han-Sung Kang
    • Gookjoo Jeong
    • Hansol Bae
    • Hyunkyung Lee
    • Seungyeon Lee
    • Sun Jung Kim
  • View Affiliations

  • Published online on: September 14, 2015     https://doi.org/10.3892/ijo.2015.3164
  • Pages: 1923-1931
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Abstract

Secretin receptor (SCTR), the G-protein coupled receptor (GPCR) for secretin, has been observed to be upregulated in a few tumor types while downregulated in others, promoting or suppressing the proliferation of tumor cells, respectively. However, little is known about the molecular regulatory mechanism of dysregulation in cancer. In the present study, an analysis of the biological pathways affected by methylation in breast cancer using the methylome databases revealed that GPCRs played a major part in the affected pathway. SCTR, one of the dysregulated GPCRs, showed hypermethylation (p<0.01) and downregulation (p<0.05) in breast cancer tissues. Pathway analysis after the downregulation of SCTR by siRNA in MCF-10A cells identified the G2/M stage checkpoint as the top-scored pathway. Cell cycle-related genes were all upregulated or downregulated suppressing cell proliferation. However, the overexpression of SCTR in MCF-7 cells led to a 35% increase of the cell proliferation index and 2.1-fold increase of cellular migration. Our findings indicate that SCTR suppresses the proliferation of normal breast cells, while the gene stimulates the proliferation and migration of cancer cells being downregulated by promoter methylation.
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November-2015
Volume 47 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kang S, Kim B, Kang H, Jeong G, Bae H, Lee H, Lee S and Kim SJ: SCTR regulates cell cycle-related genes toward anti-proliferation in normal breast cells while having pro-proliferation activity in breast cancer cells. Int J Oncol 47: 1923-1931, 2015.
APA
Kang, S., Kim, B., Kang, H., Jeong, G., Bae, H., Lee, H. ... Kim, S.J. (2015). SCTR regulates cell cycle-related genes toward anti-proliferation in normal breast cells while having pro-proliferation activity in breast cancer cells. International Journal of Oncology, 47, 1923-1931. https://doi.org/10.3892/ijo.2015.3164
MLA
Kang, S., Kim, B., Kang, H., Jeong, G., Bae, H., Lee, H., Lee, S., Kim, S. J."SCTR regulates cell cycle-related genes toward anti-proliferation in normal breast cells while having pro-proliferation activity in breast cancer cells". International Journal of Oncology 47.5 (2015): 1923-1931.
Chicago
Kang, S., Kim, B., Kang, H., Jeong, G., Bae, H., Lee, H., Lee, S., Kim, S. J."SCTR regulates cell cycle-related genes toward anti-proliferation in normal breast cells while having pro-proliferation activity in breast cancer cells". International Journal of Oncology 47, no. 5 (2015): 1923-1931. https://doi.org/10.3892/ijo.2015.3164