Cancer/testis antigen NY-SAR-35 enhances cell proliferation, migration, and invasion

Song,Myung-Ha; Kim,Ye-Rin; Lee,Jun-Won; Lee,Chang-Hun; Lee,Sang-Yull

doi:10.3892/ijo.2015.3264

Cancer/testis antigen NY-SAR-35 enhances cell proliferation, migration, and invasion

Authors:
- Myung-Ha Song
- Ye-Rin Kim
- Jun-Won Lee
- Chang-Hun Lee
- Sang-Yull Lee
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Affiliations: Department of Biochemistry, School of Medicine, Pusan National University, Yangsan, Gyeongsangnam-do 626-870, Republic of Korea, Department of Life Science and Genetic Engineering, Paichai University, Daejeon 302-735, Republic of Korea, Department of Pathology, School of Medicine, Pusan National University, Yangsan, Gyeongsangnam-do 626-870, Republic of Korea
Published online on: November 24, 2015 https://doi.org/10.3892/ijo.2015.3264
Pages: 569-576

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Abstract

The cancer/testis antigen NY-SAR-35 is aberrantly expressed in various cancer tissues and cancer cell lines but not in normal tissues except for the testis. A previous study demonstrated that the expression of NY-SAR-35 is activated by hypomethylation in cancer cells. However, the functions of this antigen remain unexplored. In the present study, we investigated the role of NY-SAR‑35 in human embryonic kidney (HEK) 293 cells using exogenous expression system of the gene. NY-SAR‑35 was predominantly expressed at the cytoplasm and was mainly observed in spermatogonia and spermatocytes. Expression of NY-SAR-35 in stable HEK293 transfectant clones was 2-fold higher than the control cells promoting cell growth and proliferation. NY-SAR-35 overexpression also enhanced cell migration and invasion ~2-fold and 4-fold more than the control, respectively. In contrast, small interfering RNA-mediated knockdown of NY-SAR-35 suppressed cell proliferation, migration, and invasion in HEK293 stable transfectants. We concluded that NY-SAR-35 as a cancer/testis antigen enhanced cell proliferation and invasion.

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