RhoA is associated with invasion and poor prognosis in colorectal cancer

Jeong,Dongjun; Park,Soyoung; Kim,Hyungjoo; Kim,Chang-Jin; Ahn,Tae  Sung; Bae,Sang  Byung; Kim,Han  Jo; Kim,Tae  Hyun; Im,Jungkyun; Lee,Moon  Soo; Kwon,Hyog  Young; Baek,Moo  Jun

doi:10.3892/ijo.2015.3281

RhoA is associated with invasion and poor prognosis in colorectal cancer

Authors:
- Dongjun Jeong
- Soyoung Park
- Hyungjoo Kim
- Chang-Jin Kim
- Tae Sung Ahn
- Sang Byung Bae
- Han Jo Kim
- Tae Hyun Kim
- Jungkyun Im
- Moon Soo Lee
- Hyog Young Kwon
- Moo Jun Baek
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Affiliations: Soonchunhyang Medical Science Research Institute, College of Medicine, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea, Department of Pathology, College of Medicine, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea, Department of Surgery, College of Medicine, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea, Department of Oncology, College of Medicine, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea, Department of Chemistry, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea, Department of Nanochemical Engineering, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea, Soonchunhyang Institute of Medi-bio Science, Soonchunhyang University, Cheonan, Chungcheongnam-do, Republic of Korea
Published online on: December 8, 2015 https://doi.org/10.3892/ijo.2015.3281
Pages: 714-722

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Abstract

Colorectal cancer is one of the most common cancers and is the fourth leading cause of cancer death in Korea. Mortality of colorectal cancer is strongly associated with the metastatic spread of the disease. As such, it is important to find and characterize signaling pathways involved in colon cancer metastasis. We investigated the functional importance of RhoA using human cell lines as well as 150 colorectal cancer patient-derived samples as it remains unclear whether RhoA functions as either an oncogene or a tumor suppressor in colon cancer. RhoA was highly expressed in metastatic cancer cell lines. Although cancer cell proliferation was only moderately impaired after depletion of RhoA, RhoA-depleted cancer cells exhibited markedly reduced migration and invasion ability in vitro. Furthermore, we found that RhoA is associated with the invasion of lymph nodes and blood vessels in the patient colorectal cancer samples. Most notably, patients with higher RhoA expression had a significantly poorer 5-year survival rate after surgery. These results suggest that RhoA is a marker of poor prognosis in colorectal cancer and may be a promising target for cancer treatment.

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