The correlation of microRNA-181a and target genes with poor prognosis of glioblastoma patients

Huang,Shi-Xiong; Zhao,Zhong-Yan; Weng,Guo-Hu; He,Xiang-Ying; Wu,Chan-Ji; Fu,Chuan-Yi; Sui,Zhi-Yan; Zhong,Xiao-Ming; Liu,Tao

doi:10.3892/ijo.2016.3511

The correlation of microRNA-181a and target genes with poor prognosis of glioblastoma patients

Authors:
- Shi-Xiong Huang
- Zhong-Yan Zhao
- Guo-Hu Weng
- Xiang-Ying He
- Chan-Ji Wu
- Chuan-Yi Fu
- Zhi-Yan Sui
- Xiao-Ming Zhong
- Tao Liu
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Affiliations: Department of Neurology, People's Hospital of Hainan Province, Haikou, Hainan 570311, P.R. China, Department of Neurosurgery, People's Hospital of Hainan Province, Haikou, Hainan 570311, P.R. China, Department of Radiology, Ganzhou City People's Hospital, Ganzhou, Jiangxi 341000, P.R. China
Published online on: May 9, 2016 https://doi.org/10.3892/ijo.2016.3511
Pages: 217-224

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Abstract

To investigate the expression and clinical significance of miR-181a and its target genes in glioblastoma multiforme (GBM), the expression levels of miR-181a and three target genes in human normal brain tissues and GBM were analyzed in silico using gene microarray, gene ontology, KEGG pathway and hierarchical clustering analysis followed by validation with quantitative RT-PCR. Our results show that miR-181a is down-regulated in GBM patients. The three target genes, ANGPT2, ARHGAP18 and LAMC1, are negatively correlated with the expression of miR-181a. Moreover, high expression of ANGPT2 or LAMC1 together with large size of GBM is correlated with a shorter median overall survival. In conclusion, our results showed that miR-181a and it targets ANGPT2 and LAMC1 might be predictors of prognosis in GBM patients.

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