Influence of doxorubicin on apoptosis and oxidative stress in breast cancer cell lines

  • Authors:
    • Nesstor Pilco-Ferreto
    • Gloria M. Calaf
  • View Affiliations

  • Published online on: June 3, 2016     https://doi.org/10.3892/ijo.2016.3558
  • Pages: 753-762
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Abstract

Breast cancer is one of the leading causes of mortality among women worldwide due to aggressive behavior, early metastasis, resistance to existing chemotherapeutic agent and high mortality rate. Doxorubicin (Dox) is a powerful antitumoral drug. It is one of the most active agents for treatment of breast cancer. The aim of the present study was to evaluate the influence of Dox in apoptosis and oxidative stress in the breast cancer cell lines MCF-10F, MCF-7 and MDA-MB-231. These studies showed that Dox decreased anti-apoptotic Bcl-2 protein expression and affected oxidative stress by increasing hydrogen peroxide production and simultaneously decreasing NF-κB gene and protein expression in MCF-7, a tumorigenic triple-positive cell line. Results also indicated that Dox induced apoptosis by upregulating Bax, caspase-8 and caspase-3 and downregulation of Bcl-2 protein expression. On the contrary, ROS damage decreased by increasing SOD2 gene and protein expression and hydrogen peroxide production with parallel NF-κB protein expression decrease in MDA-MB-231, a tumorigenic triple-negative breast cancer cell line. It can be concluded that Dox activated apoptosis by inducing proteolytic processing of Bcl-2 family, caspases and simultaneously decreased oxidative stress by influencing ROS damage in MCF-7 and MDA-MB-231 cell lines.
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August-2016
Volume 49 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Pilco-Ferreto N and Calaf GM: Influence of doxorubicin on apoptosis and oxidative stress in breast cancer cell lines. Int J Oncol 49: 753-762, 2016.
APA
Pilco-Ferreto, N., & Calaf, G.M. (2016). Influence of doxorubicin on apoptosis and oxidative stress in breast cancer cell lines. International Journal of Oncology, 49, 753-762. https://doi.org/10.3892/ijo.2016.3558
MLA
Pilco-Ferreto, N., Calaf, G. M."Influence of doxorubicin on apoptosis and oxidative stress in breast cancer cell lines". International Journal of Oncology 49.2 (2016): 753-762.
Chicago
Pilco-Ferreto, N., Calaf, G. M."Influence of doxorubicin on apoptosis and oxidative stress in breast cancer cell lines". International Journal of Oncology 49, no. 2 (2016): 753-762. https://doi.org/10.3892/ijo.2016.3558