2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside suppresses human colorectal cancer cell metastasis through inhibiting NF-κB activation

Lin,Chien-Liang; Hsieh,Shu-Ling; Leung,Wan; Jeng,Jiiang-Huei; Huang,Guan-Cheng; Lee,Chining-Ting; Wu,Chih-Chung

doi:10.3892/ijo.2016.3574

2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside suppresses human colorectal cancer cell metastasis through inhibiting NF-κB activation

Authors:
- Chien-Liang Lin
- Shu-Ling Hsieh
- Wan Leung
- Jiiang-Huei Jeng
- Guan-Cheng Huang
- Chining-Ting Lee
- Chih-Chung Wu
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Affiliations: Department of Pharmacy, Yuan's General Hospital, Kaohsiung 802, Taiwan, R.O.C., Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 811, Taiwan, R.O.C., Department of Radiology and Nuclear Medicine, Yuan's General Hospital, Kaohsiung 802, Taiwan, R.O.C., Department of Dentistry, National Taiwan University Hospital, Taipei 100, Taiwan, R.O.C., Department of Health-Business Administration, School of Nursing, Fooyin University, Kaohsiung 831, Taiwan, R.O.C., Department of Nutrition and Health Sciences, Chang Jung Christian University, Tainan 711, Taiwan, R.O.C.
Published online on: June 9, 2016 https://doi.org/10.3892/ijo.2016.3574
Pages: 629-638

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Abstract

2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (THSG), a major component of Polygonum multiflorum Thunb (He-Shou-Wu), has been reported to exhibit antioxidant and anti-inflammatory effects. However, its anti-metastatic effect against colorectal cancer is still unclear. In this study, cell migration, invasion and adhesion abilities as well as metastasis-associated protein and NF-κB pathway signaling factor expression were analyzed after treating HT-29 cells with THSG. According to the results, the migration and invasiveness of HT-29 cells were reduced after treatment with 5 or 10 mM THSG (p<0.05). Additionally, the levels of matrix metalloproteinase-2 (MMP-2) and phosphorylated VE-cadherin in HT-29 cells were reduced and the transepithelial electrical resistance (TEER) of EA.hy926 endothelial cell monolayers was increased after incubation in THSG for 24 h (p<0.05). Cell adhesion ability and the E-selectin and intercellular adhesion molecule-1 (ICAM-1) protein levels were reduced when EA.hy926 endothelial cells were treated with THSG (p<0.05). In addition, the cytoplasmic phosphorylation of IκB, the nuclear p65 level and the DNA-binding activity of NF-κB were reduced after treating HT-29 or EA.hy926 cells with 5 or 10 mM THSG (p<0.05). These results suggest that THSG inhibits HT-29 cell metastasis by suppressing cell migration, invasion and adhesion. Furthermore, THSG inhibits metastasis-associated protein expression by suppressing NF-κB pathway activation.

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