Open Access

STAT3 inhibitor, cucurbitacin I, is a novel therapeutic agent for osteosarcoma

  • Authors:
    • Toru Oi
    • Kunihiro Asanuma
    • Akihiko Matsumine
    • Takao Matsubara
    • Tomoki Nakamura
    • Takahiro Iino
    • Yumiko Asanuma
    • Mikinobu Goto
    • Kazuma Okuno
    • Takuya Kakimoto
    • Yuki Yada
    • Akihiro Sudo
  • View Affiliations

  • Published online on: November 3, 2016     https://doi.org/10.3892/ijo.2016.3757
  • Pages: 2275-2284
  • Copyright: © Oi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The development of clinical agents remains a costly and time-consuming process. Although identification of new uses of existing drugs has been recognized as a more efficient approach for drug discovery than development of novel drugs, little screening of drugs that might be used for a rare malignant tumor such as osteosarcoma (OS) has been performed. In this study, we attempted to identify new molecular targeted agents for OS by employing Screening Committee of Anticancer Drugs (SCADS) kits. To screen compounds for OS treatment, their effect on cell viability of the OS cell lines 143B, MG63, HOS, SAOS-2, and HUO9 were evaluated. Candidate drugs were narrowed down based on a global anti-proliferative effect against these five OS cell lines. After excluding cytotoxic compounds and compounds unsuitable for in vivo administration, cucurbitacin I was extracted. Cucurbitacin I has been found to have cytotoxic and anti-proliferative properties against several tumors through inhibition of signal transducer and activator of transcription 3 (STAT3) activation. Cucurbitacin I dose- and time-dependently inhibited the proliferation of all five OS cell lines. Following cucurbitacin I treatment, STAT3 was inactivated and analysis of Mcl-1, cleaved PARP and caspase-3 indicated apoptosis induction. Expression of cell cycle regulator proteins, such as phospho-cyclin D1, c-Myc and survivin, were suppressed. Finally, cucurbitacin I potently inhibited the tumor growth of human OS 143B cells in nude mice. Our in vitro and in vivo results suggest that STAT3 inhibition by cucurbitacin I will be an effective and new approach for the treatment of OS.
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December-2016
Volume 49 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Oi T, Asanuma K, Matsumine A, Matsubara T, Nakamura T, Iino T, Asanuma Y, Goto M, Okuno K, Kakimoto T, Kakimoto T, et al: STAT3 inhibitor, cucurbitacin I, is a novel therapeutic agent for osteosarcoma. Int J Oncol 49: 2275-2284, 2016.
APA
Oi, T., Asanuma, K., Matsumine, A., Matsubara, T., Nakamura, T., Iino, T. ... Sudo, A. (2016). STAT3 inhibitor, cucurbitacin I, is a novel therapeutic agent for osteosarcoma. International Journal of Oncology, 49, 2275-2284. https://doi.org/10.3892/ijo.2016.3757
MLA
Oi, T., Asanuma, K., Matsumine, A., Matsubara, T., Nakamura, T., Iino, T., Asanuma, Y., Goto, M., Okuno, K., Kakimoto, T., Yada, Y., Sudo, A."STAT3 inhibitor, cucurbitacin I, is a novel therapeutic agent for osteosarcoma". International Journal of Oncology 49.6 (2016): 2275-2284.
Chicago
Oi, T., Asanuma, K., Matsumine, A., Matsubara, T., Nakamura, T., Iino, T., Asanuma, Y., Goto, M., Okuno, K., Kakimoto, T., Yada, Y., Sudo, A."STAT3 inhibitor, cucurbitacin I, is a novel therapeutic agent for osteosarcoma". International Journal of Oncology 49, no. 6 (2016): 2275-2284. https://doi.org/10.3892/ijo.2016.3757