Expression of metallothionein 3 in ductal breast cancer

  • Authors:
    • Agnieszka Gomulkiewicz
    • Karolina Jablonska
    • Bartosz Pula
    • Jedrzej Grzegrzolka
    • Sylwia Borska
    • Marzenna Podhorska-Okolow
    • Andrzej Wojnar
    • Janusz Rys
    • Aleksandra Ambicka
    • Maciej Ugorski
    • Maciej Zabel
    • Piotr Dziegiel
  • View Affiliations

  • Published online on: November 4, 2016     https://doi.org/10.3892/ijo.2016.3759
  • Pages: 2487-2497
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Abstract

Metallothionein 3 (MT-3) has the ability to regulate the growth of nerve cells, but the significance of MT-3 expression outside the central nervous system and its participation in carcinogenesis have not yet been clarified. The aim of our study was to investigate the expression of MT-3 in ductal breast cancer and to determine its relationship with well-defined clinicopathological factors in this type of tumor. The study was conducted on 134 cases of invasive ductal breast carcinoma (IDC), 42 samples of non-malignant breast tissue (NMBT), and 26 cases of mastopathy. Moreover, selected breast cancer cell lines (MCF-7, SKBR-3, MDA-MB-231, BO2) and normal human breast epithelial cells (hTERT-HME1) were used. The expression of MT-3 was examined on the protein level using immunohistochemistry and on the mRNA level using real-time PCR. It was shown that the MT-3 protein in cells of IDC and mastopathy appeared in the cytoplasm as well as in the cell nuclei. Both the cytoplasmic and nuclear expression of MT-3 was significantly lower in IDC than in the mastopathies (p<0.0001 and p<0.001). However, no significant correlation was demonstrated between the level of MT-3 protein and the studied clinicopathological factors. The mRNA expression of MT-3 in IDC was also lower than in non‑malignant breast tissue (p<0.0001). Furthermore, in the cases of IDC with lymph node metastasis, the level of MT-3 mRNA was significantly lower than in the cases without metastasis (p=0.0199). The expression of MT-3 mRNA in breast cancer cell lines was significantly lower than in the normal human breast epithelial cell line (p<0.001). These results suggest that MT-3 may play a role in the malignant transformation of breast epithelial cells and in tumor progression.
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December-2016
Volume 49 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Gomulkiewicz A, Jablonska K, Pula B, Grzegrzolka J, Borska S, Podhorska-Okolow M, Wojnar A, Rys J, Ambicka A, Ugorski M, Ugorski M, et al: Expression of metallothionein 3 in ductal breast cancer. Int J Oncol 49: 2487-2497, 2016.
APA
Gomulkiewicz, A., Jablonska, K., Pula, B., Grzegrzolka, J., Borska, S., Podhorska-Okolow, M. ... Dziegiel, P. (2016). Expression of metallothionein 3 in ductal breast cancer. International Journal of Oncology, 49, 2487-2497. https://doi.org/10.3892/ijo.2016.3759
MLA
Gomulkiewicz, A., Jablonska, K., Pula, B., Grzegrzolka, J., Borska, S., Podhorska-Okolow, M., Wojnar, A., Rys, J., Ambicka, A., Ugorski, M., Zabel, M., Dziegiel, P."Expression of metallothionein 3 in ductal breast cancer". International Journal of Oncology 49.6 (2016): 2487-2497.
Chicago
Gomulkiewicz, A., Jablonska, K., Pula, B., Grzegrzolka, J., Borska, S., Podhorska-Okolow, M., Wojnar, A., Rys, J., Ambicka, A., Ugorski, M., Zabel, M., Dziegiel, P."Expression of metallothionein 3 in ductal breast cancer". International Journal of Oncology 49, no. 6 (2016): 2487-2497. https://doi.org/10.3892/ijo.2016.3759