MicroRNA-145 inhibits tumorigenesis and invasion of cervical cancer stem cells

Zhou,Xi; Yue,Yan; Wang,Renxiao; Gong,Baolan; Duan,Zhao

doi:10.3892/ijo.2017.3857

MicroRNA-145 inhibits tumorigenesis and invasion of cervical cancer stem cells

Authors:
- Xi Zhou
- Yan Yue
- Renxiao Wang
- Baolan Gong
- Zhao Duan
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Affiliations: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Obstetrics and Gynecology, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
Published online on: January 19, 2017 https://doi.org/10.3892/ijo.2017.3857
Pages: 853-862

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Abstract

MicroRNA (miR)-145 has been reported to induce cancer stem cell (CSC) differentiation through downregulation of the stem cell transcription factors (TFs) that maintain CSC pluripotency. High expression of miR-145 indicates a good prognosis in cancer patients, but its role in cervical cancer stem cells (CCSCs) is not known. We show that expression of miR-145 and core stem cell transcription factors, Sox2, Nanog and Oct4, are associated with the pluripotency of CCSCs, with increased expression of miR-145 after cervical tumorsphere (CT) differentiation. miR-145 overexpression inhibited expression of core TFs, as well as decreasing tumor invasion and colony formation, whereas miR-145 knockdown led to the opposite effects. Injection of adenovirus-miR-145 significantly reduced tumor growth in nude mice. High miR-145 expression predicted a better prognosis compared with that in patients with low miR-145 expression after analyses of The Cancer Genome Atlas (TCGA) data. These results suggest that miR-145 is able to induce CT differentiation through enzymolyzing TFs and might be a therapeutic target for cervical carcinoma.

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