Nicotine inhibits CD24 expression in Lewis lung carcinoma cells by upregulation of RAS expression

  • Authors:
    • Da-Hua Liu
    • Min An
    • Bai-Li Bao
    • Fu Ren
    • Pu Xia
  • View Affiliations

  • Published online on: May 22, 2018     https://doi.org/10.3892/ijo.2018.4415
  • Pages: 815-822
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Abstract

Cluster of ddifferentiation 24 (CD24) is a widely used cancer stem cell (CSC) marker in numerous cancer types. However, a number of studies have shown that CD24 is a prognostic marker, but not a CSC marker for lung adenocarcinoma. In the present study, firstly, bioinformatic analyses were used to identify the CD24 mRNA levels in the subtypes of lung cancer. Secondly, CD24high and CD24low cells were isolated from the side population of Lewis lung carcinoma (LLC) cells using flow cytometry. Furthermore, the stemness of CD24high and CD24low cells were determined in vivo and in vitro. Lastly, the mechanism(s) of nicotine-inhibited CD24 expression in LLC cells were assessed. The main findings of this study are that: i) CD24 could be used as a prognostic marker for human lung adenocarcinoma; ii) the in vitro and in vivo experiments did not determine a significant influence of CD24 on the tumorgenicity of LLC cells; and iii) nicotine inhibited CD24 expression in LLC cells by upregulation of RAS. However, the detailed mechanism(s) of these results require further analysis.
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August-2018
Volume 53 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Liu D, An M, Bao B, Ren F and Xia P: Nicotine inhibits CD24 expression in Lewis lung carcinoma cells by upregulation of RAS expression. Int J Oncol 53: 815-822, 2018.
APA
Liu, D., An, M., Bao, B., Ren, F., & Xia, P. (2018). Nicotine inhibits CD24 expression in Lewis lung carcinoma cells by upregulation of RAS expression. International Journal of Oncology, 53, 815-822. https://doi.org/10.3892/ijo.2018.4415
MLA
Liu, D., An, M., Bao, B., Ren, F., Xia, P."Nicotine inhibits CD24 expression in Lewis lung carcinoma cells by upregulation of RAS expression". International Journal of Oncology 53.2 (2018): 815-822.
Chicago
Liu, D., An, M., Bao, B., Ren, F., Xia, P."Nicotine inhibits CD24 expression in Lewis lung carcinoma cells by upregulation of RAS expression". International Journal of Oncology 53, no. 2 (2018): 815-822. https://doi.org/10.3892/ijo.2018.4415