Upregulation of miR-96 promotes radioresistance in glioblastoma cells via targeting PDCD4

  • Authors:
    • Pin Guo
    • Yanan Yu
    • Zibin Tian
    • Yingying Lin
    • Yongming Qiu
    • Weicheng Yao
    • Lijuan Zhang
  • View Affiliations

  • Published online on: July 23, 2018     https://doi.org/10.3892/ijo.2018.4498
  • Pages: 1591-1600
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Abstract

Glioblastoma multiforme (GBM) is the most deadly brain tumor, and it is characterized by extremely poor therapeutic response and overall survival. Adjuvant radiotherapy remains the standard of care following surgical resection. Thus, elucidating the mechanisms conferring radioresistance in GBM is extremely urgent. In the present study, miR-96 was demonstrated to be significantly upregulated in radioresistant GBM cells. Knockdown of miR-96 in the radioresistant GBM cells T98G elevated the % of apoptotic cells and reduced their clonogenic formation ability following radiotherapy. By contrast, overexpression of miR-96 in the radiosensitive GBM cells U87-MG reduced the % of apoptotic cells and increased their clonogenic formation ability following radiotherapy. Results from phosphorylated-H2A histone family member X (γH2AX) foci staining and comet assays revealed that miR-96 enhanced the DNA repair processes. Furthermore, miR-96 overexpression conferred radioresistance by downregulating programmed cell death protein 4 (PDCD4). Luciferase assay results revealed that miR-96 bound to the 3'UTR of PDCD4 mRNA. Finally, U87-MG cells regained radiosensitivity following PDCD4 overexpression. Taken together, the present is the first study to establish that upregulation of miR-96 in GBM cells confers radioresistance via targeting PDCD4, which might be a potential therapeutic target for GBM.
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October-2018
Volume 53 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Guo P, Yu Y, Tian Z, Lin Y, Qiu Y, Yao W and Zhang L: Upregulation of miR-96 promotes radioresistance in glioblastoma cells via targeting PDCD4. Int J Oncol 53: 1591-1600, 2018.
APA
Guo, P., Yu, Y., Tian, Z., Lin, Y., Qiu, Y., Yao, W., & Zhang, L. (2018). Upregulation of miR-96 promotes radioresistance in glioblastoma cells via targeting PDCD4. International Journal of Oncology, 53, 1591-1600. https://doi.org/10.3892/ijo.2018.4498
MLA
Guo, P., Yu, Y., Tian, Z., Lin, Y., Qiu, Y., Yao, W., Zhang, L."Upregulation of miR-96 promotes radioresistance in glioblastoma cells via targeting PDCD4". International Journal of Oncology 53.4 (2018): 1591-1600.
Chicago
Guo, P., Yu, Y., Tian, Z., Lin, Y., Qiu, Y., Yao, W., Zhang, L."Upregulation of miR-96 promotes radioresistance in glioblastoma cells via targeting PDCD4". International Journal of Oncology 53, no. 4 (2018): 1591-1600. https://doi.org/10.3892/ijo.2018.4498