Identification of COL1A1 as an invasion‑related gene in malignant astrocytoma

Sun,Shen; Wang,Yue; Wu,Yue; Gao,Yue; Li,Qi; Abdulrahman,Ayanlaja   Abiola; Liu,Xin‑Feng; Ji,Guang‑Quan; Gao,Jin; Li,Li; Wan,Fa‑Ping; Li,Yun‑Qing; Gao,Dian‑Shuai

doi:10.3892/ijo.2018.4568

Identification of COL1A1 as an invasion‑related gene in malignant astrocytoma

Authors:
- Shen Sun
- Yue Wang
- Yue Wu
- Yue Gao
- Qi Li
- Ayanlaja Abiola Abdulrahman
- Xin‑Feng Liu
- Guang‑Quan Ji
- Jin Gao
- Li Li
- Fa‑Ping Wan
- Yun‑Qing Li
- Dian‑Shuai Gao
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Affiliations: Department of Histology and Embryology, Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China, Department of Neurobiology and Anatomy, Xuzhou Key Laboratory of Neurobiology, Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China, Department of Pathophysiology, Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China, Department of Human Anatomy, Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China, Department of Anatomy and Histology, The Fourth Military Medical University, Xi'an, Shaanxi 710003, P.R. China, National Demonstration Center for Experimental Basic Medical Science Education, Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China
Published online on: September 21, 2018 https://doi.org/10.3892/ijo.2018.4568
Pages: 2542-2554
Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Malignant astrocytoma (MA) is the most common and severe type of brain tumor. A greater understanding of the underlying mechanisms responsible for the development of MA would be beneficial for the development of targeted molecular therapies. In the present study, the upregulated differentially expressed genes (DEGs) in MA were obtained from the Gene Expression Omnibus database using R/Bioconductor software. DEGs in different World Health Organization classifications were compared using the Venny tool and 15 genes, including collagen type I α1 chain (COL1A1) and laminin subunit γ1 (LAMC1), were revealed to be involved in the malignant progression of MA. In addition, the upregulated DEGs in MA were evaluated using functional annotations of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes with the Database for Annotation, Visualization, and Integrated Discovery tool. The results indicated that invasion‑associated enrichment was observed in ‘extracellular matrix’ (ECM), ‘cell adhesion’ and ‘phosphoinositide 3‑kinase‑protein kinase B signaling pathway’. Subsequently, the analysis of the protein‑protein interactions was performed using STRING and Cytoscape software, which revealed that the ECM component was the invasion‑associated module and its corresponding genes included COL1A1, LAMC1 and fibronectin 1. Finally, survival Kaplan‑Meier estimate was conducted using cBioportal online, which demonstrated that COL1A1 expression affected the survival of and recurrence in patients with MA. Moreover, the results of in vitro Transwell assay and western blot analysis revealed that the depleted levels of COL1A1 also decreased the expression of several proteins associated with cell invasion, including phosphorylated‑signal transducer and activator of transcription 3, matrix metalloproteinase (MMP)‑2, MMP‑9 and nuclear factor‑κB. On the whole, the present study identified the invasion‑related target genes and the associated potential pathways in MA. The results indicated that COL1A1 may be a candidate biomarker for the prognosis and treatment of MA.

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