Underexpression of α-1-microglobulin/bikunin precursor predicts a poor prognosis in oral squamous cell carcinoma

  • Authors:
    • Shoichi Sekikawa
    • Takeshi Onda
    • Nami Miura
    • Takeshi Nomura
    • Nobuo Takano
    • Takahiko Shibahara
    • Kazufumi Honda
  • View Affiliations

  • Published online on: October 1, 2018     https://doi.org/10.3892/ijo.2018.4581
  • Pages: 2605-2614
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Abstract

In the present study, in order to identify novel diagnostic biomarkers for the malignant behavior of oral squamous cell carcinoma (OSCC), we determined the proteomic profiles of several OSCC cell lines and keratinocytes by two-dimensional fluorescence difference gel electrophoresis and liquid chromatography tandem mass spectrometry. The protein expression level of α-1-microglobulin/bikunin precursor (AMBP) was found to be significantly lower in the OSCC cell lines than in the keratinocytes, and a significant decrease in AMBP mRNA expression was confirmed in the OSCC cell lines by RT-qPCR. To investigate the biological function of AMBP in OSCC, the cells were transiently transfected with an AMBP overexpression vector; the AMBP-overexpressing cells exhibited a significantly decreased invasion and migration in comparison to the mock-transfected control cells, although no significant changes in cell proliferation were observed. Immunohistochemistry revealed that the underexpression of AMBP was significantly associated with a high metastatic potential to cervical lymph nodes and a poor overall survival. Thus, the expression of AMBP is an independent predictive factor of cervical lymph node metastasis and a prognostic factor of overall survival, and it is involved in both cell invasion and metastasis in cervical lymph nodes in OSCC.
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December-2018
Volume 53 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sekikawa S, Onda T, Miura N, Nomura T, Takano N, Shibahara T and Honda K: Underexpression of α-1-microglobulin/bikunin precursor predicts a poor prognosis in oral squamous cell carcinoma. Int J Oncol 53: 2605-2614, 2018.
APA
Sekikawa, S., Onda, T., Miura, N., Nomura, T., Takano, N., Shibahara, T., & Honda, K. (2018). Underexpression of α-1-microglobulin/bikunin precursor predicts a poor prognosis in oral squamous cell carcinoma. International Journal of Oncology, 53, 2605-2614. https://doi.org/10.3892/ijo.2018.4581
MLA
Sekikawa, S., Onda, T., Miura, N., Nomura, T., Takano, N., Shibahara, T., Honda, K."Underexpression of α-1-microglobulin/bikunin precursor predicts a poor prognosis in oral squamous cell carcinoma". International Journal of Oncology 53.6 (2018): 2605-2614.
Chicago
Sekikawa, S., Onda, T., Miura, N., Nomura, T., Takano, N., Shibahara, T., Honda, K."Underexpression of α-1-microglobulin/bikunin precursor predicts a poor prognosis in oral squamous cell carcinoma". International Journal of Oncology 53, no. 6 (2018): 2605-2614. https://doi.org/10.3892/ijo.2018.4581