The double effect of walnut septum extract (Juglans regia L.) counteracts A172 glioblastoma cell survival and bacterial growth

Genovese,Carlo; Cambria,Maria  Teresa; D'angeli,Floriana; Addamo,Alessandro   Paolo; Malfa,Giuseppe   Antonio; Siracusa,Laura; Pulvirenti,Luana; Anfuso,Carmelina  Daniela; Lupo,Gabriella; Salmeri,Mario

doi:10.3892/ijo.2020.5130

The double effect of walnut septum extract (Juglans regia L.) counteracts A172 glioblastoma cell survival and bacterial growth

Authors:
- Carlo Genovese
- Maria Teresa Cambria
- Floriana D'angeli
- Alessandro Paolo Addamo
- Giuseppe Antonio Malfa
- Laura Siracusa
- Luana Pulvirenti
- Carmelina Daniela Anfuso
- Gabriella Lupo
- Mario Salmeri
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Affiliations: Department of Biomedical and Biotechnological Sciences, Microbiology Section, University of Catania, I‑95123, Italy, Department of Biomedical and Biotechnological Sciences, Biochemistry Section, University of Catania, I‑95123, Italy, Department of Drug Science, Biochemistry Section, University of Catania, I‑95125 Catania, Italy, Istituto di Chimica Biomolecolare del CNR, I‑95126 Catania, Italy
Published online on: October 5, 2020 https://doi.org/10.3892/ijo.2020.5130
Pages: 1129-1144
Copyright : © Genovese et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

Walnut (Juglans regia L.) is considered to be a ‘superfood’ for its multiple protective actions on human health. Walnut extracts have proven antitumor activity in different cancer cell lines. However, the efficacy of septum extract against glioblastoma has still not been investigated. Glioblastoma is the most difficult type of brain cancer to treat. The standard therapy, based on temozolomide, causes several side effects, including neutropenia and lymphocytopenia, which often favor the onset of opportunistic infections. In the present study, the chemical profile of the Sicilian walnut septum ethanolic extract was analyzed using high‑performance liquid chromatography (HPLC)‑diode array detection and HPLC‑electrospray ionization tandem mass spectrometry. The potential cytostatic activity of the extract against the human A172 glioblastoma cell line was investigated and the results showed that the extract could decrease cancer cell proliferation and migration. Using cytofluorimetric analyses and caspase‑3 assays, the pro‑apoptotic action of walnut extract was demonstrated. Furthermore, the evaluation of the antibacterial activity highlighted the efficacy of the extract in reducing Gram‑positive and Gram‑negative bacterial growth, most of which were resistant to the antibiotic, ciprofloxacin. Finally, Prediction of Activity Spectra for Substances analysis showed the predicted antitumor and antibacterial activity of HPLC detected compounds. The promising results could provide novel perspective in the field of chemotherapeutic co‑adjuvants.

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