Open Access

MEF2A is a transcription factor for circPVT1 and contributes to the malignancy of acute myeloid leukemia

  • Authors:
    • Kun Wu
    • Yuntao Li
    • Bo Nie
    • Chong Guo
    • Xiaobo Ma
    • Linyan Li
    • Shenju Cheng
    • Yanhong Li
    • Shan Luo
    • Yun Zeng
    • Jian Yu
    • Mingxia Shi
  • View Affiliations

  • Published online on: September 25, 2024     https://doi.org/10.3892/ijo.2024.5699
  • Article Number: 111
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acute myeloid leukemia (AML) is a hematological malignancy with a high relapse rate and a poor survival rate. The circular RNA circPVT1 and myocyte enhancer factor 2A (MEF2A) have unique functions in the progression of AML; however, the underlying mechanisms and clinical significance remain to be clarified. Bioinformatics and database analyses were used to assess the transcription factors and target genes of circPVT1. Dual‑luciferase reporter gene and argonaute 2‑RNA immunoprecipitation assays were used to verify the targeted relationships. The expression levels of related genes and proteins were detected by reverse transcription‑quantitative PCR and western blotting. Cell viability and apoptosis were detected by Cell Counting Kit‑8 assay and flow cytometry, respectively. The results revealed that circPVT1 was highly expressed in AML samples and cell lines, and that MEF2A regulated the expression of circPVT1. MEF2A overexpression promoted cell viability and epithelial‑mesenchymal transition (EMT), and inhibited cell apoptosis. In addition, circPVT1 was revealed to target the regulation of microRNA (miR)‑455‑3p, and miR‑455‑3p targeted the regulation of MCL1 expression, thus indicating that circPVT1 promoted MCL1 expression through its interaction with miR‑455‑3p. Furthermore, cells were transfected with the small interfering RNA‑(si)‑circPVT1, miR‑455‑3p inhibitor or si‑MCL1, and si‑circPVT1 and si‑MCL1 inhibited the viability and EMT of NB4 and HL‑60 cells. However, the miR‑455‑3p inhibitor had the opposite effect on cells. In conclusion, MEF2A may act as a transcription factor of circPVT1 to promote the malignant process of AML, and knockdown of circPVT1 could inhibit the viability and EMT of AML cells through the miR‑455‑3p/MCL1 axis.
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November-2024
Volume 65 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Wu K, Li Y, Nie B, Guo C, Ma X, Li L, Cheng S, Li Y, Luo S, Zeng Y, Zeng Y, et al: MEF2A is a transcription factor for circPVT1 and contributes to the malignancy of acute myeloid leukemia. Int J Oncol 65: 111, 2024.
APA
Wu, K., Li, Y., Nie, B., Guo, C., Ma, X., Li, L. ... Shi, M. (2024). MEF2A is a transcription factor for circPVT1 and contributes to the malignancy of acute myeloid leukemia. International Journal of Oncology, 65, 111. https://doi.org/10.3892/ijo.2024.5699
MLA
Wu, K., Li, Y., Nie, B., Guo, C., Ma, X., Li, L., Cheng, S., Li, Y., Luo, S., Zeng, Y., Yu, J., Shi, M."MEF2A is a transcription factor for circPVT1 and contributes to the malignancy of acute myeloid leukemia". International Journal of Oncology 65.5 (2024): 111.
Chicago
Wu, K., Li, Y., Nie, B., Guo, C., Ma, X., Li, L., Cheng, S., Li, Y., Luo, S., Zeng, Y., Yu, J., Shi, M."MEF2A is a transcription factor for circPVT1 and contributes to the malignancy of acute myeloid leukemia". International Journal of Oncology 65, no. 5 (2024): 111. https://doi.org/10.3892/ijo.2024.5699