search button

Ribozyme mediated cleavage of cell-associated isoform of vascular endothelial growth factor inhibits liver metastasis of a pancreatic cancer cell line

  • Authors:
    • Tetsuji Tokunaga
    • Yoshiyuki Abe
    • Takashi Tsuchida
    • Hiroyuki Hatanaka
    • Yoshiro Oshika
    • Masashi Tomisawa
    • Masumi Yoshimura
    • Yasuyuki Ohnishi
    • Hiroshi Kijima
    • Hitoshi Yamazaki
    • Yoshito Ueyama
    • Masato Nakamura

  • View Affiliations

  • Published online on: November 1, 2002     https://doi.org/10.3892/ijo.21.5.1027
  • Pages: 1027-1032
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Stromal angiogenesis is an important factor for progression of malignant neoplasms. We used hammerhead ribozymes against vascular endothelial growth factor (VEGF) gene transcripts to down-regulate cell-associated VEGF189 isoform function in the pancreatic cancer cell line MIA PaCa2. MIA PaCa2 transfected with anti-VEGF189 ribozyme did not show any alteration of growth rate under tissue culture. When the transformants were subcutaneously transplanted, tumour volume of the ribozyme-transfected MIA PaCa2 xenografts was significantly smaller (P<0.01). No metastasis of MIA PaCa2 transfected with anti-VEGF189 was apparent, while disabled ribozyme-transfected MIA PaCa2 showed significant liver metastasis (P<0.05). These results suggested that VEGF189 plays an important role in growth and metastatic potential through alteration of angiogenic balance in cancer.

Related Articles

Journal Cover

November 2002
Volume 21 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 4.5
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 9.6
  • CiteScore Rank: Q1: #66/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.88
  • SCImago Journal Rank (SJR): 1.099
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
Tokunaga T, Abe Y, Tsuchida T, Hatanaka H, Oshika Y, Tomisawa M, Yoshimura M, Ohnishi Y, Kijima H, Yamazaki H, Yamazaki H, et al: Ribozyme mediated cleavage of cell-associated isoform of vascular endothelial growth factor inhibits liver metastasis of a pancreatic cancer cell line. Int J Oncol 21: 1027-1032, 2002.
APA
Tokunaga, T., Abe, Y., Tsuchida, T., Hatanaka, H., Oshika, Y., Tomisawa, M. ... Nakamura, M. (2002). Ribozyme mediated cleavage of cell-associated isoform of vascular endothelial growth factor inhibits liver metastasis of a pancreatic cancer cell line. International Journal of Oncology, 21, 1027-1032. https://doi.org/10.3892/ijo.21.5.1027
MLA
Tokunaga, T., Abe, Y., Tsuchida, T., Hatanaka, H., Oshika, Y., Tomisawa, M., Yoshimura, M., Ohnishi, Y., Kijima, H., Yamazaki, H., Ueyama, Y., Nakamura, M."Ribozyme mediated cleavage of cell-associated isoform of vascular endothelial growth factor inhibits liver metastasis of a pancreatic cancer cell line". International Journal of Oncology 21.5 (2002): 1027-1032.
Chicago
Tokunaga, T., Abe, Y., Tsuchida, T., Hatanaka, H., Oshika, Y., Tomisawa, M., Yoshimura, M., Ohnishi, Y., Kijima, H., Yamazaki, H., Ueyama, Y., Nakamura, M."Ribozyme mediated cleavage of cell-associated isoform of vascular endothelial growth factor inhibits liver metastasis of a pancreatic cancer cell line". International Journal of Oncology 21, no. 5 (2002): 1027-1032. https://doi.org/10.3892/ijo.21.5.1027