Oridonin induces growth inhibition and apoptosis of a variety of human cancer cells

  • Authors:
    • Takayuki Ikezoe
    • Sophie S. Chen
    • Xian-Jun Tong
    • David Heber
    • Hirokuni Taguchi
    • H. Phillip Koeffler
  • View Affiliations

  • Published online on: October 1, 2003     https://doi.org/10.3892/ijo.23.4.1187
  • Pages: 1187-1193
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Abstract

PC-SPES is an eight herbal mixture that was shown to have activity against prostate cancer. Recently, we purified oridonin from Rabdosia rubescens, one component of PC-SPES, by high performance liquid chromatography (HPLC). The ability of oridonin to inhibit the proliferation of cancer cells was examined by MTT assay. Oridonin effectively inhibited the proliferation of a wide variety of cancer cells including those from prostate (LNCaP, DU145, PC3), breast (MCF-7, MDA-MB231), non-small cell lung (NSCL) (NCI-H520, NCI-H460, NCI-H1299) cancers, acute promyelocytic leukemia (NB4), and glioblastoma multiforme (U118, U138) with ED50s ranging from 1.8 to 7.5 µg/ml. TUNEL assay and cell cycle analysis showed that oridonin induced apoptosis and G0/G1 cell cycle arrest in LNCaP prostate cancer cells. In addition, expression of p21waf1 was induced in LNCaP and NCI-H520 cells in a p53-dependent manner. Interestingly, when p53 was suppressed by over-expression of E6 from human papilloma virus type 16 (HPV-16), these cells lost their sensitivity to oridonin-induced growth inhibition and apoptosis. Taken together, oridonin inhibited the proliferation of cancer cells via apoptosis and cell cycle arrest with p53 playing a central role in several cancer types which express the wild-type p53 gene. Oridonin may be a novel, adjunctive therapy for a large variety of malignancies and probably represents one of the major, active components of PC-SPES.

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October 2003
Volume 23 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ikezoe T, Chen SS, Tong X, Heber D, Taguchi H and Koeffler HP: Oridonin induces growth inhibition and apoptosis of a variety of human cancer cells. Int J Oncol 23: 1187-1193, 2003.
APA
Ikezoe, T., Chen, S.S., Tong, X., Heber, D., Taguchi, H., & Koeffler, H.P. (2003). Oridonin induces growth inhibition and apoptosis of a variety of human cancer cells. International Journal of Oncology, 23, 1187-1193. https://doi.org/10.3892/ijo.23.4.1187
MLA
Ikezoe, T., Chen, S. S., Tong, X., Heber, D., Taguchi, H., Koeffler, H. P."Oridonin induces growth inhibition and apoptosis of a variety of human cancer cells". International Journal of Oncology 23.4 (2003): 1187-1193.
Chicago
Ikezoe, T., Chen, S. S., Tong, X., Heber, D., Taguchi, H., Koeffler, H. P."Oridonin induces growth inhibition and apoptosis of a variety of human cancer cells". International Journal of Oncology 23, no. 4 (2003): 1187-1193. https://doi.org/10.3892/ijo.23.4.1187