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Nuclear texture and chromatin structure in OV1/VCR human multidrug-resistant cell line

  • Authors:
    • Sonia Yatouji
    • Aurelie Trussardi-Regnier
    • Chantal Trentesaux
    • Françoise Liautaud-Roger
    • Jean Dufer

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  • Published online on: October 1, 2003     https://doi.org/10.3892/ijo.23.4.1225
  • Pages: 1225-1230
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Abstract

Previous studies have demonstrated that multidrug-resistant leukemic cells displayed nuclear texture changes. In this work, the human ovarian carcinoma cell line IGROV1 and its multidrug-resistant variant OV1/VCR were studied. Cell smears of these cell populations were analysed by image cytometry. As compared to sensitive cells, OV1/VCR display a chromatin global decondensation as assessed by textural features analysis. In order to correlate this decondensation with alterations in chromatin structure, DNase I was used. OV1/VCR DNA displayed an increased DNase I sensitivity, suggesting an increased chromatin accessibility. Furthermore, OV1/VCR cells displayed an increased level in acetylated histone H4, a mechanism known to be associated with transcriptionally active chromatin and relaxed chromatin conformation.

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Journal Cover

October 2003
Volume 23 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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  • Journal Metrics
  • Impact Factor: 4.5
  • Ranked Oncology
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  • CiteScore: 9.6
  • CiteScore Rank: Q1: #66/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.88
  • SCImago Journal Rank (SJR): 1.099
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Spandidos Publications style
Yatouji S, Trussardi-Regnier A, Trentesaux C, Liautaud-Roger F and Dufer J: Nuclear texture and chromatin structure in OV1/VCR human multidrug-resistant cell line. Int J Oncol 23: 1225-1230, 2003.
APA
Yatouji, S., Trussardi-Regnier, A., Trentesaux, C., Liautaud-Roger, F., & Dufer, J. (2003). Nuclear texture and chromatin structure in OV1/VCR human multidrug-resistant cell line. International Journal of Oncology, 23, 1225-1230. https://doi.org/10.3892/ijo.23.4.1225
MLA
Yatouji, S., Trussardi-Regnier, A., Trentesaux, C., Liautaud-Roger, F., Dufer, J."Nuclear texture and chromatin structure in OV1/VCR human multidrug-resistant cell line". International Journal of Oncology 23.4 (2003): 1225-1230.
Chicago
Yatouji, S., Trussardi-Regnier, A., Trentesaux, C., Liautaud-Roger, F., Dufer, J."Nuclear texture and chromatin structure in OV1/VCR human multidrug-resistant cell line". International Journal of Oncology 23, no. 4 (2003): 1225-1230. https://doi.org/10.3892/ijo.23.4.1225