Relationship between activation of epidermal growth factor receptor and cell dissociation in pancreatic cancer

  • Authors:
    • Xiaodong Tan
    • Hiroshi Egami
    • Shinji Ishikawa
    • Masahide Nakagawa
    • Takatoshi Ishiko
    • Hidenobu Kamohara
    • Masahiko Hirota
    • Michio Ogawa
  • View Affiliations

  • Published online on: November 1, 2004     https://doi.org/10.3892/ijo.25.5.1303
  • Pages: 1303-1309
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In our previous investigations, mitogen-activated protein kinase kinase 2 (MEK2)/extracellular signal-regulated kinase 2 (ERK2) signaling pathway was found to be correlated with the cell dissociation induced by dissociation factor (DF) in pancreatic cancer cells. In this study, the expressions of epidermal growth factor receptor (EGFR), phosphorylated EGFR (p-EGFR), and its downstream kinases MEK1/2 and ERK1/2, were analyzed to clarify the regulatory mechanism of cell dissociation in pancreatic cancer cells. Two hamster (PC-1.0 and PC-1) and two human (AsPC-1 and Capan-2) pancreatic cancer cell lines were used. Immunocytochemical study was performed using anti-EGFR, p-EGFR, phosphorylated MEK1/2 (p-MEK1/2), and phosphorylated ERK1/2 (p-ERK1/2) antibodies. DF-treatment markedly induced the expressions of EGFR, p-EGFR, p-MEK1/2, p-ERK1/2, as well as the dissociation of cell colonies in PC-1 and Capan-2 cells. In contrast, AG1478 (an EGFR inhibitor) treatment significantly induced the cell aggregation in PC-1.0 and AsPC-1 cells which usually grew as single cells, but strongly suppressed the expressions of EGFR, p-EGFR, p-MEK1/2, and p-ERK1/2. These observations demonstrate that activation of EGFR is closely involved in cell dissociation in pancreatic cancer through activating MEK/ERK signaling pathway.

Related Articles

Journal Cover

November 2004
Volume 25 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Tan X, Egami H, Ishikawa S, Nakagawa M, Ishiko T, Kamohara H, Hirota M and Ogawa M: Relationship between activation of epidermal growth factor receptor and cell dissociation in pancreatic cancer. Int J Oncol 25: 1303-1309, 2004.
APA
Tan, X., Egami, H., Ishikawa, S., Nakagawa, M., Ishiko, T., Kamohara, H. ... Ogawa, M. (2004). Relationship between activation of epidermal growth factor receptor and cell dissociation in pancreatic cancer. International Journal of Oncology, 25, 1303-1309. https://doi.org/10.3892/ijo.25.5.1303
MLA
Tan, X., Egami, H., Ishikawa, S., Nakagawa, M., Ishiko, T., Kamohara, H., Hirota, M., Ogawa, M."Relationship between activation of epidermal growth factor receptor and cell dissociation in pancreatic cancer". International Journal of Oncology 25.5 (2004): 1303-1309.
Chicago
Tan, X., Egami, H., Ishikawa, S., Nakagawa, M., Ishiko, T., Kamohara, H., Hirota, M., Ogawa, M."Relationship between activation of epidermal growth factor receptor and cell dissociation in pancreatic cancer". International Journal of Oncology 25, no. 5 (2004): 1303-1309. https://doi.org/10.3892/ijo.25.5.1303