Antitumor activity of cationic liposome-mediated Bax gene transfer in osteosarcoma cells: Induction of apoptosis and caspase-independent cell death

  • Authors:
    • Kenya Okumura
    • Minoru Nakase
    • Shinnosuke Nakamura
    • Madoka Inui
    • Kenichi Hiramoto
    • Toshiro Tagawa
  • View Affiliations

  • Published online on: August 1, 2005     https://doi.org/10.3892/ijo.27.2.433
  • Pages: 433-438
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Abstract

The purpose of this study was to evaluate the anti-tumor effects of osteosarcoma (HOSM-1) cells via transfer of the Bax gene using a cationic liposome. We evaluated the levels of Bax, Bcl-xL, Bcl-2 and cytochrome c expression by Western blot analysis, and caspase-9 and -3 activities were determined in a colorimetric assay. Apoptosis was detected using a TUNEL assay, and cell growth inhibition was determined in an MTT assay. Following Bax gene transfer, release of cytochrome c to the cytosol was detected, the activities of caspase-9 and -3 increased, and TUNEL-positive cells (37.5%) were detected. Cell survival rate was 50.8% under these conditions. Induction of apoptosis was inhibited by a caspase inhibitor (zVAD-fmk), but only a slight increase in cell survival rate occurred. Hence, since not only apoptosis but also caspase-independent cell death is induced in HOSM-1 cells, we anticipate that Bax gene therapy with cationic liposomes will be useful for osteosarcoma.

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August 2005
Volume 27 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Okumura K, Nakase M, Nakamura S, Inui M, Hiramoto K and Tagawa T: Antitumor activity of cationic liposome-mediated Bax gene transfer in osteosarcoma cells: Induction of apoptosis and caspase-independent cell death. Int J Oncol 27: 433-438, 2005.
APA
Okumura, K., Nakase, M., Nakamura, S., Inui, M., Hiramoto, K., & Tagawa, T. (2005). Antitumor activity of cationic liposome-mediated Bax gene transfer in osteosarcoma cells: Induction of apoptosis and caspase-independent cell death. International Journal of Oncology, 27, 433-438. https://doi.org/10.3892/ijo.27.2.433
MLA
Okumura, K., Nakase, M., Nakamura, S., Inui, M., Hiramoto, K., Tagawa, T."Antitumor activity of cationic liposome-mediated Bax gene transfer in osteosarcoma cells: Induction of apoptosis and caspase-independent cell death". International Journal of Oncology 27.2 (2005): 433-438.
Chicago
Okumura, K., Nakase, M., Nakamura, S., Inui, M., Hiramoto, K., Tagawa, T."Antitumor activity of cationic liposome-mediated Bax gene transfer in osteosarcoma cells: Induction of apoptosis and caspase-independent cell death". International Journal of Oncology 27, no. 2 (2005): 433-438. https://doi.org/10.3892/ijo.27.2.433