Heparanase, TrkC and p75NTR: Their functional involvement in human medulloblastoma cell invasion

  • Authors:
    • Neeta D. Sinnappah-Kang
    • Adam J. Kaiser
    • Bryan E. Blust
    • Robert E. Mrak
    • Dario Marchetti
  • View Affiliations

  • Published online on: September 1, 2005     https://doi.org/10.3892/ijo.27.3.617
  • Pages: 617-626
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Abstract

Medulloblastoma is the most common malignant childhood brain tumor. Although some previous reports have shown up to a 70% 5-year survival for some of these patients, it is at the cost of significant long-term treatment-related morbidity. The cellular mechanisms leading to metastatic disease in medulloblastoma are mainly unknown. For the first time, we demonstrate the differential expression of heparanase in medulloblastomas and how these differences at the mRNA and protein levels affect the activity and invasive properties of three newly developed cell lines. Furthermore, heparanase expression was confirmed in 7 (88%) of 8 medulloblastoma clinical samples by immunohistochemical staining. Heparanase was found to be localized in the cytoplasm and nucleus. Quantitative polymerase chain reaction revealed a negative correlation between heparanase and TrkC (which is associated with a favorable clinical outcome). The activation of TrkC or TrkC/p75NTR by NT-3 affected heparanase activity and cell-invasive properties of medulloblastoma cells in vitro. Taken together, our data extend the body of evidence that invasion and expression/functionality of heparanase, in a context linked to TrkC and p75NTR, may play critical roles in the disease progression of medulloblastoma.

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September 2005
Volume 27 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sinnappah-Kang ND, Kaiser AJ, Blust BE, Mrak RE and Marchetti D: Heparanase, TrkC and p75NTR: Their functional involvement in human medulloblastoma cell invasion. Int J Oncol 27: 617-626, 2005.
APA
Sinnappah-Kang, N.D., Kaiser, A.J., Blust, B.E., Mrak, R.E., & Marchetti, D. (2005). Heparanase, TrkC and p75NTR: Their functional involvement in human medulloblastoma cell invasion. International Journal of Oncology, 27, 617-626. https://doi.org/10.3892/ijo.27.3.617
MLA
Sinnappah-Kang, N. D., Kaiser, A. J., Blust, B. E., Mrak, R. E., Marchetti, D."Heparanase, TrkC and p75NTR: Their functional involvement in human medulloblastoma cell invasion". International Journal of Oncology 27.3 (2005): 617-626.
Chicago
Sinnappah-Kang, N. D., Kaiser, A. J., Blust, B. E., Mrak, R. E., Marchetti, D."Heparanase, TrkC and p75NTR: Their functional involvement in human medulloblastoma cell invasion". International Journal of Oncology 27, no. 3 (2005): 617-626. https://doi.org/10.3892/ijo.27.3.617