Inhibitory effects of ascorbic acid on AP-1 activity and transformation of JB6 cells

  • Authors:
    • C Huang
    • W Ma
    • Z Dong
  • View Affiliations

  • Published online on: February 1, 1996     https://doi.org/10.3892/ijo.8.2.389
  • Pages: 389-393
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Ascorbic acid (vitamin C) has been reported as an anti-cancer agent. Previous in vitro studies using either primary cell cultures from cancer patients or tumor cell lines have indicated that different kinds of tumor cells may have different sensitivities to ascorbic acid for the inhibition of tumorigeneity or growth. Because the JB6 mouse epidermal cell system has been used extensively as an in vitro model for the study of tumor promotion and progression, we assessed the effects of ascorbic acid on transformation in JB6 cell variants. The results show that ascorbic acid could inhibit 5.5% to 97.1% of transformation of JB6 P+ cell Cl 41-19 induced by TPA, EGF or EGF + insulin, but has no effect on anchorage-independent growth of JB6 transformed cell A33. Since our previous results indicated that induced AP-1 activity is required for tumor promoter induced-transformation, we tested whether inhibition of tumor promoter-induced transformation by ascorbic acid is through an AP-1 inhibition mechanism. Our results indicated that ascorbic acid inhibited AP-I activity at the same dose range for inhibition of transformation. These results demonstrated that ascorbic acid has inhibitory effects on JB6 cell tumor promoter-induced transformation, but no influence on tumor cell phenotype expression and provided the basic knowledge for understanding of vitamin C action on tumor prevention.

Related Articles

Journal Cover

February 1996
Volume 8 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Huang C, Ma W and Dong Z: Inhibitory effects of ascorbic acid on AP-1 activity and transformation of JB6 cells. Int J Oncol 8: 389-393, 1996.
APA
Huang, C., Ma, W., & Dong, Z. (1996). Inhibitory effects of ascorbic acid on AP-1 activity and transformation of JB6 cells. International Journal of Oncology, 8, 389-393. https://doi.org/10.3892/ijo.8.2.389
MLA
Huang, C., Ma, W., Dong, Z."Inhibitory effects of ascorbic acid on AP-1 activity and transformation of JB6 cells". International Journal of Oncology 8.2 (1996): 389-393.
Chicago
Huang, C., Ma, W., Dong, Z."Inhibitory effects of ascorbic acid on AP-1 activity and transformation of JB6 cells". International Journal of Oncology 8, no. 2 (1996): 389-393. https://doi.org/10.3892/ijo.8.2.389