In order to examine how the adenovirus-mediated gene therapy induce anti-adenoviral immunity, we set up experiments for a murine cancer model. We first administered adenovirus carrying LacZ (AxCALacZ) gene to DBA/2 mice intraperitoneally, then two weeks after administration of the virus, P815 tumor cells infected with adenovirus carrying LacZ gene were inoculated intraperitoneally to the AxCALacZ immune mice. The mice rejected the P815 tumor infected with AxCALacZ and survived for long periods. The peritoneal T cells in adenovirus immune mice had a strong killing effect of adenovirus-infected P815 cells. Further, parental P815 tumor cells were inoculated into the mice, which rejected the P815 tumor infected with AxCALacZ. All mice rejected P815 tumor cells and survived for long periods. These mice developed P815 specific tumor immunity, which was confirmed by a cytotoxicity assay.

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