Downregulation of expression of transporters associated with antigen processing 1 and 2 and human leukocyte antigen I and its effect on immunity in nasopharyngeal carcinoma patients

Ren,Yan‑Xin; Yang,Jie; Zhang,Li‑Juan; Sun,Rui‑Mei; Zhao,Liu‑Fang; Zhang,Ming; Chen,Yun; Ma,Jing; Qiao,Kun; Sun,Qiang‑Ming; Long,Hai‑Ting; Huang,Yun‑Chao; Li,Xiao‑Jiang

doi:10.3892/mco.2013.194

Downregulation of expression of transporters associated with antigen processing 1 and 2 and human leukocyte antigen I and its effect on immunity in nasopharyngeal carcinoma patients

Authors:
- Yan‑Xin Ren
- Jie Yang
- Li‑Juan Zhang
- Rui‑Mei Sun
- Liu‑Fang Zhao
- Ming Zhang
- Yun Chen
- Jing Ma
- Kun Qiao
- Qiang‑Ming Sun
- Hai‑Ting Long
- Yun‑Chao Huang
- Xiao‑Jiang Li
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Affiliations: Head and Neck Tumor Research Center, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650118, P.R. China, Department of Pathology, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650118, P.R. China, Department of Radiotherapy, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650118, P.R. China, Department of Otolaryngology, Kunming Children's Hospital, Kunming, Yunnan 650034, P.R. China, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, Yunnan 650118, P.R. China, Department of Cardiothoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650118, P.R. China
Published online on: September 26, 2013 https://doi.org/10.3892/mco.2013.194
Pages: 51-58

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Abstract

The human leukocyte antigen (HLA)‑I and antigen‑processing machinery (APM) are crucial in the anticancer immune response. The aim of this study was to assess the clinical significance of the APM components [transporters associated with antigen processing (TAP)-1 and ‑2 and HLA‑I] in nasopharyngeal carcinoma (NPC). A total of 58 NPC specimens and 20 healthy specimens used as control were evaluated by semiquantitative immunohistochemistry for three APM components (TAP‑1, TAP‑2 and HLA‑I). The expression of the APM components in NPC was downregulated. CD4+ and CD8+ T cells were measured by flow cytometry and IL‑10 was measured by ELISA. The number of CD8+ T cells and the expression of IL‑10 were higher and the number of CD4+ T cells was lower in NPC, compared to the controls. The number of CD8+ T cells and the expression of IL‑10 were negatively correlated with TAP‑1, TAP‑2 and HLA‑I expression. The clinical phase, lymph node metastasis, distant metastasis, pathological type, TAP‑1 expression, TAP‑2 expression and HLA‑I expression were identified as prognostic factors by the Kaplan‑Meier analysis. A multivariate analysis using a Cox regression model indicated that distant metastasis and the downregulation of HLA‑Ⅰ expression were independent unfavorable prognostic factors. In conclusion, the lower expression of HLA‑I induced immunosuppression in NPC patients and was associated with a poor prognosis.

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