Enhanced antitumor effect of combining chemotherapy with Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes in mice with EBV-related non-Hodgkin's lymphoma

  • Authors:
    • Rui Deng
    • Hai Yi
    • Yi‑Lan Liu
    • Fang‑Yi Fan
    • Li Fu
    • Ye‑Cheng Li
    • Guo‑Shun Li
    • Si‑Han Lai
    • Xiao‑Juan Miao
    • Yan‑Rong Shuai
    • Guang‑Cui He
    • Yi Wang
    • Yan Zeng
    • Hao‑Ping Sun
    • Ling Qiu
    • Yi Su
  • View Affiliations

  • Published online on: September 16, 2015     https://doi.org/10.3892/mco.2015.646
  • Pages: 1233-1238
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Abstract

Epstein-Barr virus (EBV)-related non‑Hodgkin's lymphoma (NHL) represents a major problem in hematological clinical studies due to its drug tolerance and refractoriness. EBV infection is a key factor driving the process of tumor growth. Immune therapy is an important biotherapeutic method of treating cancer, which is attracting increasing attention. We hypothesized that combining conventional chemotherapy with immune therapy in the treatment of EBV‑related NHL may achieve better outcomes. First, we successfully cloned large numbers of EBV‑specific T cells by immune stimulation ex vivo. Subsequently, the combined therapy was applied in a murine model of human EBV‑related NHL. As expected, combined therapy inhibited tumor growth more effectively compared with monotherapy. In addition, we continuously tested the tumor‑associated immune microenvironment and observed that the numbers of tumor‑infiltrating cytotoxic T lymphocytes (CTLs) and macrophages were elevated following combined therapy. These effects suggest that EBV‑specific CTLs may indirectly promote an innate immune reaction in lymphoma by activating tumor‑infiltrating macrophage proliferation. Our findings may provide a guide for the prospective treatment of EBV-related NHL.
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November-2015
Volume 3 Issue 6

Print ISSN: 2049-9450
Online ISSN:2049-9469

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Spandidos Publications style
Deng R, Yi H, Liu YL, Fan FY, Fu L, Li YC, Li GS, Lai SH, Miao XJ, Shuai YR, Shuai YR, et al: Enhanced antitumor effect of combining chemotherapy with Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes in mice with EBV-related non-Hodgkin's lymphoma. Mol Clin Oncol 3: 1233-1238, 2015
APA
Deng, R., Yi, H., Liu, Y., Fan, F., Fu, L., Li, Y. ... Su, Y. (2015). Enhanced antitumor effect of combining chemotherapy with Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes in mice with EBV-related non-Hodgkin's lymphoma. Molecular and Clinical Oncology, 3, 1233-1238. https://doi.org/10.3892/mco.2015.646
MLA
Deng, R., Yi, H., Liu, Y., Fan, F., Fu, L., Li, Y., Li, G., Lai, S., Miao, X., Shuai, Y., He, G., Wang, Y., Zeng, Y., Sun, H., Qiu, L., Su, Y."Enhanced antitumor effect of combining chemotherapy with Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes in mice with EBV-related non-Hodgkin's lymphoma". Molecular and Clinical Oncology 3.6 (2015): 1233-1238.
Chicago
Deng, R., Yi, H., Liu, Y., Fan, F., Fu, L., Li, Y., Li, G., Lai, S., Miao, X., Shuai, Y., He, G., Wang, Y., Zeng, Y., Sun, H., Qiu, L., Su, Y."Enhanced antitumor effect of combining chemotherapy with Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes in mice with EBV-related non-Hodgkin's lymphoma". Molecular and Clinical Oncology 3, no. 6 (2015): 1233-1238. https://doi.org/10.3892/mco.2015.646