Effect of nutrient starvation on proliferation and cytokine secretion of peripheral blood lymphocytes

Ota,Yoshiko; Ishihara,Soichiro; Otani,Kensuke; Yasuda,Koji; Nishikawa,Takeshi; Tanaka,Toshiaki; Tanaka,Junichiro; Kiyomatsu,Tomomichi; Kawai,Kazushige; Hata,Keisuke; Nozawa,Hiroaki; Kazama,Shinsuke; Yamaguchi,Hironori; Sunami,Eiji; Kitayama,Joji; Watanabe,Toshiaki

doi:10.3892/mco.2016.763

April-2016 Volume 4 Issue 4

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April-2016 Volume 4 Issue 4

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Article

Effect of nutrient starvation on proliferation and cytokine secretion of peripheral blood lymphocytes

Authors:
- Yoshiko Ota
- Soichiro Ishihara
- Kensuke Otani
- Koji Yasuda
- Takeshi Nishikawa
- Toshiaki Tanaka
- Junichiro Tanaka
- Tomomichi Kiyomatsu
- Kazushige Kawai
- Keisuke Hata
- Hiroaki Nozawa
- Shinsuke Kazama
- Hironori Yamaguchi
- Eiji Sunami
- Joji Kitayama
- Toshiaki Watanabe
View Affiliations / Copyright

Affiliations: Department of Surgical Oncology, The University of Tokyo Hospital, Tokyo 113‑8655, Japan
Pages: 607-610
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Published online on: February 3, 2016

https://doi.org/10.3892/mco.2016.763
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Abstract

Proliferating cancer cells are exposed to nutrient deprivation. Numerous previous studies have demonstrated how nutrient deprivation affects cancer cells; however, immune cells exposed to the identical conditions have not been completely examined. Furthermore, T‑helper 2 lymphocyte predominance in certain neoplastic diseases has been reported; however, the mechanism remains unclear. The present study aimed to confirm whether nutrient deprivation affected proliferation and cytokine secretion of peripheral blood lymphocytes (PBLs). The proliferation of PBLs from healthy donors, cultured in a medium containing various glucose levels, was assessed by 3‑(4,5‑dimethylthiazol-2‑yl)-5-(3‑carboxymethoxyphenyl)-2‑(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay. The expression levels of interleukin (IL)‑4 and interferon (IFN)‑γ among CD4(+) T cells, cultured with or without glucose and activated with phorbol 12‑myristate 13‑acetate and ionomycin, were examined using an intracellular cytokine staining method. The proliferation of PBLs cultured in a medium containing <100 mg/dl glucose of the standard blood sugar (BS) level was significantly reduced compared with the proliferation observed in a medium containing a standard BS level or higher. PBLs cultured in a glucose‑free medium contained a significantly higher percentage of IL‑4‑positive and a lower percentage of IFN‑γ‑positive CD4(+) T cells compared with those cultured in a high‑glucose medium. Nutrient deprivation suppressed the proliferation of PBLs, fostered the secretion of IL‑4 and reduced secretion of IFN‑γ. It is therefore possible that glucose‑deficient microenvironments in local cancer tissues cause a partial immunodeficiency, which is advantageous to cancer growth.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of nutrient starvation on proliferation and cytokine secretion of peripheral blood lymphocytes

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Abstract

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