Mutation of the nm23-H1 gene has a non-dominant role in colorectal adenocarcinoma

Jin,Yueling; Dai,Zhensheng

doi:10.3892/mco.2016.889

Mutation of the nm23-H1 gene has a non-dominant role in colorectal adenocarcinoma

Authors:
- Yueling Jin
- Zhensheng Dai
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Affiliations: Department of Pathology, Shanghai University of Medicine and Health Sciences, Shanghai 200237, P.R. China, Department of Hematology‑Oncology, Shanghai Pudong Hospital Affiliated to Fudan University, Shanghai 201399, P.R. China
Published online on: May 9, 2016 https://doi.org/10.3892/mco.2016.889
Pages: 107-110

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Abstract

Nm23-H1 is a metastasis suppressor gene, which is has a reduced expression in patients with digestive system cancer. However, the mechanistic basis for the genetic instability remains unknown. To study the expression of the nm23‑H1 gene in patients with colorectal cancer, polymerase chain reaction‑single strand conformation polymorphism was used to analyze any point mutation, and immunohistochemistry was used to detect the expression of nm23‑H1. Results revealed that all 63 specimens of Chinese human colorectal cancer tissues exhibit no point mutation. Among those 63 specimens, 19 (30%) exhibited positive immunostaining for the nm23‑H1 protein and 44 (70%) exhibited negative immunostaining. These observations suggested that the protein and gene expression levels of nm23‑H1 are reduced in colorectal cancer compared with the adjacent normal tissues, and the point mutation in the nm23‑H1 gene is not the dominant cause of metastatic colorectal cancer.

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