Intratumoral hemorrhage-related differences in the expression of vascular endothelial growth factor, basic fibroblast growth factor and thioredoxin reductase 1 in human glioblastoma

Kaya,Bulent; Çiçek,Onur; Erdi,Fatih; Findik,Siddika; Karatas,Yasar; Esen,Hasan; Keskin,Fatih; Kalkan,Erdal

doi:10.3892/mco.2016.974

Intratumoral hemorrhage-related differences in the expression of vascular endothelial growth factor, basic fibroblast growth factor and thioredoxin reductase 1 in human glioblastoma

Authors:
- Bulent Kaya
- Onur Çiçek
- Fatih Erdi
- Siddika Findik
- Yasar Karatas
- Hasan Esen
- Fatih Keskin
- Erdal Kalkan
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Affiliations: Department of Neurosurgery, Meram Faculty of Medicine, Necmettin Erbakan University, Meram, Konya 42080, Turkey, Department of Neurosurgery, Ministry of Health, Konya Numune Hospital, Selçuklu, Konya 42060, Turkey, Department of Pathology, Meram Faculty of Medicine, Necmettin Erbakan University, Meram, Konya 42080, Turkey
Published online on: August 2, 2016 https://doi.org/10.3892/mco.2016.974
Pages: 343-346

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Abstract

The present study was designed to evaluate the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and thioredoxin reductase 1 (TrxR1) in glioblastoma multiforme (GBM) with and without intratumoral hemorrhage. Surgically resected human GBM samples from 20 patients who underwent surgery at our institute were extracted from the histopathological specimens and divided into two groups. A total of 10 samples from each type of GBM (World Health Organization grade IV, intratumoral hemorrhage‑positive or ‑negative) were included in each group. VEGF, bFGF and TrxR1 expression was analyzed using immunohistochemistry and the results were compared between groups. VEGF and bFGF immunoreactivity was significantly higher in GBMs containing intratumoral hemorrhage. Furthermore, VEGF, bFGF and TrxR1 immunointensity was significantly higher in GBMs containing intratumoral hemorrhage. Thus, the present study demonstrated a higher VEGF, bFGF and TrxR1 expression in GBMs contain intratumoral hemorrhage, indicatiogn a role of VEGF, bFGF and TrxR1 expression in the promotion of tumoral angiogenesis and tumoral growth by complex mechanisms that require further elucidation.

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