Plasma interleukin-8 levels are persistently elevated for 1 month after minimally invasive colorectal resection for colorectal cancer

Shantha Kumara,H. M.C.; Sutton,Elli; Bellini,Geoffrey  A.; Yan,Xiaohong; Rn,Vesna  Cekic; Gandhi,Nipa  Dilip; Whelan,Richard  L.

doi:10.3892/mco.2017.1538

Plasma interleukin-8 levels are persistently elevated for 1 month after minimally invasive colorectal resection for colorectal cancer

Authors:
- H. M.C. Shantha Kumara
- Elli Sutton
- Geoffrey A. Bellini
- Xiaohong Yan
- Vesna Cekic Rn
- Nipa Dilip Gandhi
- Richard L. Whelan
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Affiliations: Division of Colon and Rectal Surgery, Department of Surgery, Mount Sinai West Hospital, New York, NY 10019, USA
Published online on: December 18, 2017 https://doi.org/10.3892/mco.2017.1538
Pages: 471-476

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Abstract

Minimally invasive colorectal resection (MICR) for colorectal cancer (CRC) is associated with elevated levels of seven proangiogenic proteins that persist for 2‑4 weeks after surgery. The proangiogenic plasma may promote tumor growth postoperatively in patients with residual cancer. To the best of our knowledge, the impact of surgery on interleukin 8 (IL-8) levels is unknown. The aim of the present study was to evaluate plasma IL-8 levels after MICR for CRC. Patients with CRC enrolled in an institutional review board‑approved plasma/data bank who underwent MICR were eligible. Blood samples were taken preoperatively (preop) and at multiple postoperative (postop) time points, and were stored at ‑80˚C. Only patients for whom preop, postop day (POD) 1, POD 3 and at least 1 late postop plasma samples (POD7‑34) available were enrolled. Clinical, demographical and pathological data were collected. IL-8 levels were determined via ELISA and results were reported as the mean and ± standard deviation. The Wilcoxon signed rank test was used for analysis with P<0.05 used as the significance threshold. A total of 73 CRC patients (colon, 62%; rectal, 38%) who underwent MICR (laparoscopic‑assisted, 60%; hand‑assisted, 40%) were studied. The mean preop IL-8 level was 20.4±10.6 pg/ml. Significant elevations in plasma IL-8 levels were noted compared with preop levels on POD1 (43.1±38.6; n=72; P<0.0001), POD 3 (33.0±30.1; n=71; P<0.0001), POD7‑13 (29.9±21.9; n=50; P<0.0001), POD14‑20 (33.1±18.3; n=24; P=0.002), and for the POD21‑27 time point (24.0±9.2; n=16; P=0.002). In conclusion, plasma IL‑8 levels were significantly elevated from baseline for 4 weeks after MICR for CRC. In conjunction with the other proangiogenic MICR‑associated blood compositional changes, increased IL‑8 levels may promote tumor angiogenesis and growth postop.

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