Open Access

Single‑cell RNA‑seq necroptosis‑related genes predict the prognosis of breast cancer and affect the differentiation of CD4+ T cells in tumor immune microenvironment

  • Authors:
    • Li Guo
    • Xiuzhen Ma
    • Hong Li
    • Shuxun Yan
    • Kai Zhang
    • Jinping Li
  • View Affiliations

  • Published online on: May 28, 2024     https://doi.org/10.3892/mco.2024.2747
  • Article Number: 49
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Breast cancer (BC) is one of the most prevalent types of malignancy and a major cause of cancer‑related death. The purpose of the present study was to identify prognostic models of necroptosis‑related genes (NRGs) in BC at the single‑cell RNA‑sequencing level and reveal the role of NRGs in tumour immune microenvironment (TIME). A risk model was constructed based on Cox regression and LASSO methods. Next, high‑scoring cell populations were searched through AUCell scores, and cell subtypes were then analyzed by pseudotime analysis. Finally, the expression level of the model genes was verified by reverse transcription‑quantitative (RT‑qPCR). A new prognostic model was constructed and validated based on five NRGs (BCL2, BIRC3, AIFM1, IFNG and VDAC1), which could effectively predict the prognosis of patients with BC. NRGs were found to be highly active in CD4+ T cells and differentially expressed in their developmental trajectories. Finally, the RT‑qPCR results showed that most of the model genes were significantly overexpressed in MDA‑MB‑231 and MCF‑7 cells (P<0.05). In conclusion, an NRG signature with excellent predictive properties in prognosis and TIME was successfully established. Moreover, NRGs were involved in the differentiation and development of CD4+ T cells in TIME. These findings provide potential therapeutic strategies for BC.
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July-2024
Volume 21 Issue 1

Print ISSN: 2049-9450
Online ISSN:2049-9469

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Spandidos Publications style
Guo L, Ma X, Li H, Yan S, Zhang K and Li J: Single‑cell RNA‑seq necroptosis‑related genes predict the prognosis of breast cancer and affect the differentiation of CD4<sup>+</sup> T cells in tumor immune microenvironment. Mol Clin Oncol 21: 49, 2024
APA
Guo, L., Ma, X., Li, H., Yan, S., Zhang, K., & Li, J. (2024). Single‑cell RNA‑seq necroptosis‑related genes predict the prognosis of breast cancer and affect the differentiation of CD4<sup>+</sup> T cells in tumor immune microenvironment. Molecular and Clinical Oncology, 21, 49. https://doi.org/10.3892/mco.2024.2747
MLA
Guo, L., Ma, X., Li, H., Yan, S., Zhang, K., Li, J."Single‑cell RNA‑seq necroptosis‑related genes predict the prognosis of breast cancer and affect the differentiation of CD4<sup>+</sup> T cells in tumor immune microenvironment". Molecular and Clinical Oncology 21.1 (2024): 49.
Chicago
Guo, L., Ma, X., Li, H., Yan, S., Zhang, K., Li, J."Single‑cell RNA‑seq necroptosis‑related genes predict the prognosis of breast cancer and affect the differentiation of CD4<sup>+</sup> T cells in tumor immune microenvironment". Molecular and Clinical Oncology 21, no. 1 (2024): 49. https://doi.org/10.3892/mco.2024.2747