Screening for differential methylation status in fetal myocardial tissue samples with ventricular septal defects by promoter methylation microarrays

Zhu,Chun; Yu,Zhang-Bin; Chen,Xiao-Hui; Pan,Ya; Dong,Xiao‑Yue; Qian,Ling-Mei; Han,Shu-Ping

doi:10.3892/mmr.2010.407

Screening for differential methylation status in fetal myocardial tissue samples with ventricular septal defects by promoter methylation microarrays

Authors:
- Chun Zhu
- Zhang-Bin Yu
- Xiao-Hui Chen
- Ya Pan
- Xiao‑Yue Dong
- Ling-Mei Qian
- Shu-Ping Han
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Affiliations: Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, P.R. China, Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, P.R. China, Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, P.R. China
Published online on: November 30, 2010 https://doi.org/10.3892/mmr.2010.407
Pages: 137-143

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Abstract

To identify and provide a global assessment of DNA methylation in fetal ventricular septal defect (VSD), genomic DNA extracted from fetal myocardial tissue samples with VSD (n=21) and from normal fetal myocardial tissue samples (n=15) was analyzed for gene methylation using array‑based technology. Furthermore, the KIAA0310, RAB43, SIVA1 and NDRG2 genes were randomly selected for validation analysis using methylation-specific PCR. Our results revealed that 70 and 85 genes were regulated by hypermethylation and hypomethylation, respectively, in VSD. Different clusters of genes were associated with functions including embryo development, signal transduction, cell apoptosis and cell proliferation. In conclusion, this study identified a set of candidate genes whose expression is regulated by DNA methylation in fetal VSD.