Butylated hydroxyanisole affects immunomodulation and promotes macrophage phagocytosis in normal BALB/c mice

Hung,Fang-Ming; Chuang,Ying-Ying; Lee,Ching-Sung; Chen,Yung-Liang; Yang,Jai-Sing; Lin,Jen-Jyh; Lu,Kung-Wen; Huang,Hui-Ying; Yu,Chien-Chih; Lu,Hsu-Feng; Chung,Jing-Gung

doi:10.3892/mmr.2011.704

Butylated hydroxyanisole affects immunomodulation and promotes macrophage phagocytosis in normal BALB/c mice

Authors:
- Fang-Ming Hung
- Ying-Ying Chuang
- Ching-Sung Lee
- Yung-Liang Chen
- Jai-Sing Yang
- Jen-Jyh Lin
- Kung-Wen Lu
- Hui-Ying Huang
- Chien-Chih Yu
- Hsu-Feng Lu
- Jing-Gung Chung
View Affiliations

Affiliations: Department of Surgical Intensive Care Unit, Far Eastern Memorial Hospital, New Taipei 220, Taiwan, R.O.C., Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei 112, Taiwan, R.O.C., Department of Restaurant, Hotel and Institutional Management, Fu-Jen Catholic University, New Taipei 242, Taiwan, R.O.C., Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsinchu 300, Taiwan, R.O.C., Department of Pharmacology, China Medical University, Taichung 404, Taiwan, R.O.C., Graduate Institute of Chinese Medicine, China Medical University, Taichung 404, Taiwan, R.O.C., School of Post-Baccalaureate Chinese Medicine, China Medical University, Taichung 404, Taiwan, R.O.C., Department of Nutrition, China Medical University, Taichung 404, Taiwan, R.O.C., School of Pharmacy, China Medical University, Taichung 404, Taiwan, R.O.C., Department of Biological Science and Technology, China Medical University, Taichung 40402, Taiwan, R.O.C.
Published online on: December 12, 2011 https://doi.org/10.3892/mmr.2011.704
Pages: 683-687

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Abstract

Butylated hydroxyanisole (BHA), a synthetic antioxidant, has been used in fat and fatty foods to prevent oxidative deterioration. However, the functions of BHA on immune responses in normal mice remain elusive. The aim of the present study was to investigate the effects of oral treatment of BHA on immune responses in normal mice in vivo. BALB/c mice received various treatments. Blood samples were collected and analyzed. Flow cytometry was used to determine the levels of the cell markers. Results showed that BHA did not significantly affect the weight of the animal body and spleen in normal mice. BHA promoted macrophage phagocytosis from peripheral blood mononuclear cells, but did not alter this process in the peritoneal cavity. Furthermore, BHA did not influence natural-killer cell cytotoxicity in normal mice. Notably, BHA promoted the levels of CD3 (T cells) and decreased the level of CD19 (B cells), but did not significantly affect the levels of CD11b (monocytes) and macrophages (Mac-3) in normal mice. Based on these observations it can be concluded that BHA promotes immune responses by increasing T cells and activating phagocytosis by macrophages in normal mice. However, the molecular mechanisms require further investigation.