Changes in the expression of cardiac mitofusin-2 in different stages of diabetes in rats

  • Authors:
    • Qin Gao
    • Xiao-Mei Wang
    • Hong-Wei Ye
    • Ying Yu
    • Pin-Fang Kang
    • Hong-Ju Wang
    • Su-Dong Guan
    • Zheng-Hong Li
  • View Affiliations

  • Published online on: July 23, 2012     https://doi.org/10.3892/mmr.2012.1002
  • Pages: 811-814
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Abstract

The aim of this study was to investigate the role of mitofusin-2 (Mfn2) in different stages of diabetes in rats and to analyze the related mechanism(s). A diabetic model in SD rats was induced by a single intraperitoneal injection of 55 mg/kg streptozoticin (STZ). The hearts were isolated from diabetes mellitus (DM) rats at the fourth week (DM4W), eighth week (DM8W) and twelfth week (DM12W) and fasting blood glucose (FBG) levels and the ratio of heart weight to body weight (HW/BW) were measured. Malondialdehyde (MDA) content, superoxide dismutase (SOD) and caspase 3 activities were measured. The expression of Mfn2 of the left anterior myocardium at the mRNA level was detected using RT‑PCR. In contrast to the normal group, in the DM4W, DM8W and DM12W groups, there was a significant increase in the FBG levels, but no difference among the DM4W, DM8W and DM12W groups. The HW/BW ratio as well as the MDA content were increased, while SOD activity was reduced. Caspase‑3 activity was increased, while the expression of Mfn-2 mRNA levels was reduced. In addition, with the development of diabetic cardiomyopathy, the contents of MDA and caspase 3 were increased, whereas SOD activity and Mfn-2 mRNA levels were further reduced. In conclusion, our results indicated that with the development of diabetes, the expression of cardiac Mfn2 has showed a decrease, which may be associated with the decrease of antioxidant ability and progression of apoptosis.
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October 2012
Volume 6 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Gao Q, Wang X, Ye H, Yu Y, Kang P, Wang H, Guan S and Li Z: Changes in the expression of cardiac mitofusin-2 in different stages of diabetes in rats. Mol Med Rep 6: 811-814, 2012.
APA
Gao, Q., Wang, X., Ye, H., Yu, Y., Kang, P., Wang, H. ... Li, Z. (2012). Changes in the expression of cardiac mitofusin-2 in different stages of diabetes in rats. Molecular Medicine Reports, 6, 811-814. https://doi.org/10.3892/mmr.2012.1002
MLA
Gao, Q., Wang, X., Ye, H., Yu, Y., Kang, P., Wang, H., Guan, S., Li, Z."Changes in the expression of cardiac mitofusin-2 in different stages of diabetes in rats". Molecular Medicine Reports 6.4 (2012): 811-814.
Chicago
Gao, Q., Wang, X., Ye, H., Yu, Y., Kang, P., Wang, H., Guan, S., Li, Z."Changes in the expression of cardiac mitofusin-2 in different stages of diabetes in rats". Molecular Medicine Reports 6, no. 4 (2012): 811-814. https://doi.org/10.3892/mmr.2012.1002