Protective effects of glutamine in a rat model of endotoxemia

  • Authors:
    • Ya-Juan Zhao
    • Hong Wang
    • Xin Liu
    • Mei Sun
    • Haginoya Kazuhiro
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  • Published online on: July 26, 2012     https://doi.org/10.3892/mmr.2012.1007
  • Pages: 739-744
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Abstract

The aim of this study was to characterize the protective effects of glutamine (Gln) on brain cells undergoing experimental endotoxemia induced by lipopolysaccharide (LPS) injection. Young rats were injected with LPS or control, and a subset of LPS-injected rats were pretreated with Gln. Electron microscopy and immunohistochemistry were used to visualize apoptosis and to determine distribution and expression of nuclear factor-κB (NF-κB), heat shock protein 70 (HSP70), platelet-derived growth factor-B (PDGF-B) and PDGF receptor-β (PDGFR-β). The levels of HSP70, PDGF-B and PDGFR-β in the rat brain were comparatively analyzed by western blotting. In a rat brain model of endotoxemia, Gln decreases the magnitude of apoptosis, upregulates the expression of HSP70 and inhibits the translocation of NF-κB from the cytoplasm to the nucleus. Gln upregulates PDGF-B and PDGFR-β expression in early and advanced sepsis. PDGF-B and PDGFR-β upregulation in the cerebral cortex are likely neuroprotective effects of Gln. We found that Gln is capable of regulating the immunological defense of local brain tissue, which provides a theoretical basis for using Gln to prevent and treat encephalopathy.
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October 2012
Volume 6 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhao Y, Wang H, Liu X, Sun M and Kazuhiro H: Protective effects of glutamine in a rat model of endotoxemia. Mol Med Rep 6: 739-744, 2012.
APA
Zhao, Y., Wang, H., Liu, X., Sun, M., & Kazuhiro, H. (2012). Protective effects of glutamine in a rat model of endotoxemia. Molecular Medicine Reports, 6, 739-744. https://doi.org/10.3892/mmr.2012.1007
MLA
Zhao, Y., Wang, H., Liu, X., Sun, M., Kazuhiro, H."Protective effects of glutamine in a rat model of endotoxemia". Molecular Medicine Reports 6.4 (2012): 739-744.
Chicago
Zhao, Y., Wang, H., Liu, X., Sun, M., Kazuhiro, H."Protective effects of glutamine in a rat model of endotoxemia". Molecular Medicine Reports 6, no. 4 (2012): 739-744. https://doi.org/10.3892/mmr.2012.1007