Knockdown of RAB25 promotes autophagy and inhibits cell growth in ovarian cancer cells

  • Authors:
    • Yingtao Liu
    • Xiang Tao
    • Luoqi Jia
    • Kwai Wa Cheng
    • Yiling Lu
    • Yinhua Yu
    • Youji Feng
  • View Affiliations

  • Published online on: August 28, 2012     https://doi.org/10.3892/mmr.2012.1052
  • Pages: 1006-1012
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Abstract

RAB25 belongs to the Rab family of small GTPases and is implicated in the development of various types of human cancer. To evaluate the role of RAB25 in ovarian cancer, RAB25 was knocked down by siRNA in HEY and ES‑2 human ovarian cancer cells. Autophagy, cell growth and cell apoptosis were evaluated. The results showed that knockdown of RAB25 increased acidic vesicle organelles and GFP-microtubule-associated protein 1 light chain 3 punctate fluorescence in ovarian cancer cells. Autophagy that promoted by knockdown of RAB25 was not observed in cells where the ERK1/2 signaling pathway had been inhibited by U0126. Knockdown of RAB25 reduced cell cycle progression and cell growth. Apoptosis of ovarian cancer cells could be induced by knockdown of RAB25. These results support the tumorigenic role of RAB25 in ovarian cancer cells.
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November 2012
Volume 6 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu Y, Tao X, Jia L, Cheng KW, Lu Y, Yu Y and Feng Y: Knockdown of RAB25 promotes autophagy and inhibits cell growth in ovarian cancer cells. Mol Med Rep 6: 1006-1012, 2012.
APA
Liu, Y., Tao, X., Jia, L., Cheng, K.W., Lu, Y., Yu, Y., & Feng, Y. (2012). Knockdown of RAB25 promotes autophagy and inhibits cell growth in ovarian cancer cells. Molecular Medicine Reports, 6, 1006-1012. https://doi.org/10.3892/mmr.2012.1052
MLA
Liu, Y., Tao, X., Jia, L., Cheng, K. W., Lu, Y., Yu, Y., Feng, Y."Knockdown of RAB25 promotes autophagy and inhibits cell growth in ovarian cancer cells". Molecular Medicine Reports 6.5 (2012): 1006-1012.
Chicago
Liu, Y., Tao, X., Jia, L., Cheng, K. W., Lu, Y., Yu, Y., Feng, Y."Knockdown of RAB25 promotes autophagy and inhibits cell growth in ovarian cancer cells". Molecular Medicine Reports 6, no. 5 (2012): 1006-1012. https://doi.org/10.3892/mmr.2012.1052