Sulforaphane inhibits proliferation by causing cell cycle arrest at the G2/M phase in rabbit articular chondrocytes

  • Authors:
    • Hee-Jeong Jeong
    • Seon-Mi Yu
    • Jae-Chang Jung
    • Song-Ja Kim
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  • Published online on: August 30, 2012     https://doi.org/10.3892/mmr.2012.1057
  • Pages: 1199-1203
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Abstract

Sulforaphane (SFN), a natural compound extracted from cruciferous vegetables, exhibits potent anticancer activity in various types of tumor cells. However, the effect of SFN on the proliferation of chondrocytes is not well understood. In the present study, we addressed the mechanism of action by which SFN suppresses proliferation. We demonstrate that SFN causes an irreversible arrest in cell proliferation, as determined by trypan blue dye exclusion assay and flow cytometric analysis. SFN induced cell cycle arrest at the G2/M phase by downregulation of cyclin B, cdc2 and cdc25c and upregulation of p21WAF1/CIP1 and p53 in a dose- and time-dependent manner, as determined by western blot analysis. Our data suggest that SFN regulates cell cycle arrest at the G2/M phase in rabbit articular chondrocytes.
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November 2012
Volume 6 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Jeong H, Yu S, Jung J and Kim S: Sulforaphane inhibits proliferation by causing cell cycle arrest at the G2/M phase in rabbit articular chondrocytes. Mol Med Rep 6: 1199-1203, 2012.
APA
Jeong, H., Yu, S., Jung, J., & Kim, S. (2012). Sulforaphane inhibits proliferation by causing cell cycle arrest at the G2/M phase in rabbit articular chondrocytes. Molecular Medicine Reports, 6, 1199-1203. https://doi.org/10.3892/mmr.2012.1057
MLA
Jeong, H., Yu, S., Jung, J., Kim, S."Sulforaphane inhibits proliferation by causing cell cycle arrest at the G2/M phase in rabbit articular chondrocytes". Molecular Medicine Reports 6.5 (2012): 1199-1203.
Chicago
Jeong, H., Yu, S., Jung, J., Kim, S."Sulforaphane inhibits proliferation by causing cell cycle arrest at the G2/M phase in rabbit articular chondrocytes". Molecular Medicine Reports 6, no. 5 (2012): 1199-1203. https://doi.org/10.3892/mmr.2012.1057