A simple and practical method that prepares high molecular weight DNA ladders

  • Authors:
    • Jun-He Zhang
    • Rui Yang
    • Tian-Yun Wang
    • Wei-Hua Dong
    • Fang Wang
    • Li Wang
  • View Affiliations

  • Published online on: September 4, 2012     https://doi.org/10.3892/mmr.2012.1061
  • Pages: 1211-1213
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Abstract

The purpose of the current study was to report a simple and practical method to prepare high molecular weight (mw) DNA ladders. The method involves 1,000-4,000-base pairs (bp) DNA fragments being amplified by polymerase chain reaction (PCR), using λ DNA as a template. The constructed plasmids are digested by restriction endonucleases to produce 5-, 6-, 8- and 10-kb DNA fragments, followed by purification and precipitation with ethanol, and mixed proportionally. The 1,000-4,000-bp DNA fragments were successfully generated by PCR and 5-, 6-, 8- and 10-kb DNA fragments were obtained through the digestion of the plasmids. The bands of the prepared high mw DNA ladder were clear and may aid future molecular biology studies.
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November 2012
Volume 6 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Zhang J, Yang R, Wang T, Dong W, Wang F and Wang L: A simple and practical method that prepares high molecular weight DNA ladders. Mol Med Rep 6: 1211-1213, 2012
APA
Zhang, J., Yang, R., Wang, T., Dong, W., Wang, F., & Wang, L. (2012). A simple and practical method that prepares high molecular weight DNA ladders. Molecular Medicine Reports, 6, 1211-1213. https://doi.org/10.3892/mmr.2012.1061
MLA
Zhang, J., Yang, R., Wang, T., Dong, W., Wang, F., Wang, L."A simple and practical method that prepares high molecular weight DNA ladders". Molecular Medicine Reports 6.5 (2012): 1211-1213.
Chicago
Zhang, J., Yang, R., Wang, T., Dong, W., Wang, F., Wang, L."A simple and practical method that prepares high molecular weight DNA ladders". Molecular Medicine Reports 6, no. 5 (2012): 1211-1213. https://doi.org/10.3892/mmr.2012.1061