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December 2012 Volume 6 Issue 6

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Article

Glucose-regulated protein 75 overexpression attenuates ionizing radiation-mediated injury in PC12 cells by inducing the expression of topoisomerase IIα

  • Authors:
    • Weiwei Guo
    • Ling Yang
    • Hua Li
    • Zuojun Xie
    • Wen Liu
    • Ji Zuo
  • View Affiliations / Copyright

    Affiliations: Department of Cellular and Genetic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, P.R. China, Department of Biology, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, P.R. China, Experimental Teaching Center, Shanghai Medical College, Fudan University, Shanghai 200032, P.R. China
  • Pages: 1423-1427
    |
    Published online on: September 10, 2012
       https://doi.org/10.3892/mmr.2012.1070
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Abstract

Glucose-regulated protein 75 (Grp75), also known as mortalin/mthsp70/PBP74, is a member of the heat-shock protein 70 (HSP70) family. Grp75 is known to protect cells from stress-induced injury. Previous studies have shown that the expression of Grp75 is upregulated by a low dose of ionizing radiation (IR). To evaluate the protective role of Grp75 on cell proliferation in response to IR, Grp75 was overexpressed in a rat adrenal pheochromocytoma cell line (PC12). It was revealed that Grp75 overexpression desensitized PC12 cells to IR-mediated cell injury. In addition, the expression of topoisomerase IIα (Topo IIα) was downregulated in PC12 cells following γ-ray IR. The effect of Grp75 overexpression on Topo IIα expression was examined. It was revealed that Grp75 overexpression reversed the inhibitory effect of IR on Topo IIα expression. In conclusion, the data indicated that Grp75 overexpression attenuates IR-induced injury in PC12 cells by maintaining the expression of Topo IIα.
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Copy and paste a formatted citation
Spandidos Publications style
Guo W, Yang L, Li H, Xie Z, Liu W and Zuo J: Glucose-regulated protein 75 overexpression attenuates ionizing radiation-mediated injury in PC12 cells by inducing the expression of topoisomerase IIα. Mol Med Rep 6: 1423-1427, 2012.
APA
Guo, W., Yang, L., Li, H., Xie, Z., Liu, W., & Zuo, J. (2012). Glucose-regulated protein 75 overexpression attenuates ionizing radiation-mediated injury in PC12 cells by inducing the expression of topoisomerase IIα. Molecular Medicine Reports, 6, 1423-1427. https://doi.org/10.3892/mmr.2012.1070
MLA
Guo, W., Yang, L., Li, H., Xie, Z., Liu, W., Zuo, J."Glucose-regulated protein 75 overexpression attenuates ionizing radiation-mediated injury in PC12 cells by inducing the expression of topoisomerase IIα". Molecular Medicine Reports 6.6 (2012): 1423-1427.
Chicago
Guo, W., Yang, L., Li, H., Xie, Z., Liu, W., Zuo, J."Glucose-regulated protein 75 overexpression attenuates ionizing radiation-mediated injury in PC12 cells by inducing the expression of topoisomerase IIα". Molecular Medicine Reports 6, no. 6 (2012): 1423-1427. https://doi.org/10.3892/mmr.2012.1070
Copy and paste a formatted citation
x
Spandidos Publications style
Guo W, Yang L, Li H, Xie Z, Liu W and Zuo J: Glucose-regulated protein 75 overexpression attenuates ionizing radiation-mediated injury in PC12 cells by inducing the expression of topoisomerase IIα. Mol Med Rep 6: 1423-1427, 2012.
APA
Guo, W., Yang, L., Li, H., Xie, Z., Liu, W., & Zuo, J. (2012). Glucose-regulated protein 75 overexpression attenuates ionizing radiation-mediated injury in PC12 cells by inducing the expression of topoisomerase IIα. Molecular Medicine Reports, 6, 1423-1427. https://doi.org/10.3892/mmr.2012.1070
MLA
Guo, W., Yang, L., Li, H., Xie, Z., Liu, W., Zuo, J."Glucose-regulated protein 75 overexpression attenuates ionizing radiation-mediated injury in PC12 cells by inducing the expression of topoisomerase IIα". Molecular Medicine Reports 6.6 (2012): 1423-1427.
Chicago
Guo, W., Yang, L., Li, H., Xie, Z., Liu, W., Zuo, J."Glucose-regulated protein 75 overexpression attenuates ionizing radiation-mediated injury in PC12 cells by inducing the expression of topoisomerase IIα". Molecular Medicine Reports 6, no. 6 (2012): 1423-1427. https://doi.org/10.3892/mmr.2012.1070
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