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Molecular Medicine Reports
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December 2012 Volume 6 Issue 6

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Article

Annexin A5 protein expression is associated with the histological differentiation of uterine cervical squamous cell carcinoma in patients with an increased serum concentration

  • Authors:
    • Xin Li
    • Long Chen
    • Xiu Jun Liang
    • Yu Feng Gao
    • Xiao Jie Wang
    • Qian Xu
    • Yong Yan
    • Fu Lu Gao
  • View Affiliations / Copyright

    Affiliations: Basic Research Institution, Chengde Medical College, Chengde, P.R. China, College of Life Sciences, Hebei Normal University, Shijiazhuang, P.R. China
  • Pages: 1249-1254
    |
    Published online on: September 12, 2012
       https://doi.org/10.3892/mmr.2012.1078
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Abstract

Annexin A5 (ANXA5) is a calcium-dependent phospholipid-binding protein belonging to the annexin family and is expressed abnormally in several types of carcinoma. In the present study, ANXA5 protein expression was evaluated by western blot analysis in a series of 60 human uterine cervical squamous cell carcinomas (UCSCCs) to search for molecular alterations that may be able to serve as useful diagnostic/prognostic markers. The upregulation of ANXA5 expression was observed in 48/60 UCSCC cases (80%), whereas a weak expression was observed in the 25 normal uterine cervical tissues. ANXA5 expression was also analyzed by immunohistochemical staining, western blot and reverse transcription-polymerase chain reaction (RT-PCR) assays of the UCSCC and uterine cervical normal tissue lesions. All dysplastic tissues showed significantly increased ANXA5 expression compared with the weak signal observed in normal epithelia. A close association was observed between the ANXA5 expression levels and the histological grade of UCSCC. Compared with moderately and well-differentiated tumors, there was a significant increase in ANXA5 expression in poorly differentiated tumors. Furthermore, ANXA5 concentrations in the blood serum of the patients were significantly increased. Our findings clearly identify ANXA5 as an effective differentiation marker for the histopathological grading of UCSCCs and for the detection of epithelial dysplasia. The results from our study support the critical role of ANXA5 in the molecular profiling of UCSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Chen L, Liang XJ, Gao YF, Wang XJ, Xu Q, Yan Y and Gao FL: Annexin A5 protein expression is associated with the histological differentiation of uterine cervical squamous cell carcinoma in patients with an increased serum concentration. Mol Med Rep 6: 1249-1254, 2012.
APA
Li, X., Chen, L., Liang, X.J., Gao, Y.F., Wang, X.J., Xu, Q. ... Gao, F.L. (2012). Annexin A5 protein expression is associated with the histological differentiation of uterine cervical squamous cell carcinoma in patients with an increased serum concentration. Molecular Medicine Reports, 6, 1249-1254. https://doi.org/10.3892/mmr.2012.1078
MLA
Li, X., Chen, L., Liang, X. J., Gao, Y. F., Wang, X. J., Xu, Q., Yan, Y., Gao, F. L."Annexin A5 protein expression is associated with the histological differentiation of uterine cervical squamous cell carcinoma in patients with an increased serum concentration". Molecular Medicine Reports 6.6 (2012): 1249-1254.
Chicago
Li, X., Chen, L., Liang, X. J., Gao, Y. F., Wang, X. J., Xu, Q., Yan, Y., Gao, F. L."Annexin A5 protein expression is associated with the histological differentiation of uterine cervical squamous cell carcinoma in patients with an increased serum concentration". Molecular Medicine Reports 6, no. 6 (2012): 1249-1254. https://doi.org/10.3892/mmr.2012.1078
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Chen L, Liang XJ, Gao YF, Wang XJ, Xu Q, Yan Y and Gao FL: Annexin A5 protein expression is associated with the histological differentiation of uterine cervical squamous cell carcinoma in patients with an increased serum concentration. Mol Med Rep 6: 1249-1254, 2012.
APA
Li, X., Chen, L., Liang, X.J., Gao, Y.F., Wang, X.J., Xu, Q. ... Gao, F.L. (2012). Annexin A5 protein expression is associated with the histological differentiation of uterine cervical squamous cell carcinoma in patients with an increased serum concentration. Molecular Medicine Reports, 6, 1249-1254. https://doi.org/10.3892/mmr.2012.1078
MLA
Li, X., Chen, L., Liang, X. J., Gao, Y. F., Wang, X. J., Xu, Q., Yan, Y., Gao, F. L."Annexin A5 protein expression is associated with the histological differentiation of uterine cervical squamous cell carcinoma in patients with an increased serum concentration". Molecular Medicine Reports 6.6 (2012): 1249-1254.
Chicago
Li, X., Chen, L., Liang, X. J., Gao, Y. F., Wang, X. J., Xu, Q., Yan, Y., Gao, F. L."Annexin A5 protein expression is associated with the histological differentiation of uterine cervical squamous cell carcinoma in patients with an increased serum concentration". Molecular Medicine Reports 6, no. 6 (2012): 1249-1254. https://doi.org/10.3892/mmr.2012.1078
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